Detailed information of TA system TA036587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3631824..3632365
• Replicon: chromosome
• Accession: NZ_AP022848
• Organism: Bosea sp. ANAM02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OCUBac02_RS17455
• Protein ID: WP_173047406.1
• Coordinates: 3632042..3632365 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6F8UZZ2
• Locus tag: OCUBac02_RS17450
• Protein ID: WP_047580514.1
• Coordinates: 3631824..3632045 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUBac02_RS17430	3627407..3628309	-	903	WP_173047402.1	ABC transporter permease subunit	-
OCUBac02_RS17435	3628557..3629711	-	1155	WP_173049654.1	ABC transporter ATP-binding protein	-
OCUBac02_RS17440	3629738..3630829	-	1092	WP_173049656.1	polyamine ABC transporter substrate-binding protein	-
OCUBac02_RS17445	3630997..3631752	+	756	WP_173047404.1	DUF3750 domain-containing protein	-
OCUBac02_RS17450	3631824..3632045	+	222	WP_047580514.1	antitoxin MazE family protein	Antitoxin
OCUBac02_RS17455	3632042..3632365	+	324	WP_173047406.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OCUBac02_RS17460	3632533..3633816	-	1284	WP_047574021.1	cystathionine gamma-synthase family protein	-
OCUBac02_RS17465	3633950..3634420	+	471	WP_173047408.1	Lrp/AsnC family transcriptional regulator	-
OCUBac02_RS17470	3634421..3635428	-	1008	WP_173047410.1	alpha/beta hydrolase	-
OCUBac02_RS17475	3635563..3636714	-	1152	WP_173047412.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (7-68)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11459.48 Da
• Isoelectric Point: 6.2722

>T36587 WP_173047406.1 NZ_AP022848:3632042-3632365 [Bosea sp. ANAM02]
MKRGDLVTVALAGDFGKPRPALIVQADPFDLTATVTLLLISSDLVDAPLIRLTVEPNATNGLRLASQIMVDKAMTVRRER
IGQVFGSLDPETMIAVNRSLALFLGLG

Nucleotide

• Length: 324 bp

>T36587 NZ_AP022848:3632042-3632365 [Bosea sp. ANAM02]
ATGAAGCGCGGCGACCTCGTCACCGTCGCGCTCGCCGGGGATTTCGGGAAGCCGCGCCCTGCCCTGATCGTTCAGGCCGA
TCCGTTCGATCTGACGGCGACGGTGACTCTGCTGCTCATCTCCAGCGATCTCGTCGATGCGCCGCTGATCCGGCTGACAG
TCGAGCCCAATGCCACGAACGGCCTGCGCCTTGCCTCCCAGATCATGGTCGACAAGGCGATGACCGTGCGCCGGGAGCGG
ATCGGACAAGTTTTCGGCAGTCTCGATCCGGAAACGATGATTGCGGTGAATCGGTCGCTGGCGCTGTTCCTGGGCCTCGG
TTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8289.37 Da
• Isoelectric Point: 9.6132

>AT36587 WP_047580514.1 NZ_AP022848:3631824-3632045 [Bosea sp. ANAM02]
MASSIARRVQKRRDALRAAGLRPVQIWVPDTRRPGFSEECRRQARLVAVSDRADRDLDAFLDAALDDLDRAPE

Nucleotide

• Length: 222 bp

>AT36587 NZ_AP022848:3631824-3632045 [Bosea sp. ANAM02]
ATGGCCAGTTCCATTGCCAGACGCGTCCAGAAGCGCCGCGACGCCTTGCGGGCTGCGGGCCTGCGGCCGGTGCAGATCTG
GGTTCCCGATACGCGACGCCCCGGGTTCAGCGAGGAATGCCGACGCCAGGCGCGCCTCGTCGCAGTCAGTGACCGTGCCG
ATCGGGACCTGGATGCATTCCTTGACGCTGCGCTGGACGATCTGGACCGCGCCCCTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6F8UZZ2