Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 250187..250823 Replicon chromosome
Accession NZ_AP022842
Organism Bacillus sp. KH172YL63

Toxin (Protein)


Gene name mazF Uniprot ID A0A366ENG2
Locus tag KH172YL63_RS01350 Protein ID WP_034763063.1
Coordinates 250473..250823 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0V8H6B8
Locus tag KH172YL63_RS01345 Protein ID WP_032085355.1
Coordinates 250187..250468 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH172YL63_RS01325 246360..246944 - 585 WP_173104439.1 rhomboid family intramembrane serine protease -
KH172YL63_RS01330 247079..247429 + 351 WP_173104440.1 holo-ACP synthase -
KH172YL63_RS01335 247656..248669 + 1014 WP_173104441.1 outer membrane lipoprotein carrier protein LolA -
KH172YL63_RS01340 248855..250033 + 1179 WP_173104442.1 alanine racemase -
KH172YL63_RS01345 250187..250468 + 282 WP_032085355.1 hypothetical protein Antitoxin
KH172YL63_RS01350 250473..250823 + 351 WP_034763063.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KH172YL63_RS01355 251193..252023 + 831 WP_173104443.1 STAS domain-containing protein -
KH172YL63_RS01360 252014..252376 + 363 WP_152968625.1 STAS domain-containing protein -
KH172YL63_RS01365 252379..252780 + 402 WP_044340428.1 anti-sigma regulatory factor -
KH172YL63_RS01370 252791..253801 + 1011 WP_173104444.1 PP2C family protein-serine/threonine phosphatase -
KH172YL63_RS01375 253880..254212 + 333 WP_173104445.1 anti-sigma factor antagonist -
KH172YL63_RS01380 254209..254679 + 471 WP_173104446.1 anti-sigma B factor RsbW -
KH172YL63_RS01385 254657..255454 + 798 WP_173104447.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13005.05 Da        Isoelectric Point: 5.1554

>T36569 WP_034763063.1 NZ_AP022842:250473-250823 [Bacillus sp. KH172YL63]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTIIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDDALQISVGLIQF

Download         Length: 351 bp

>T36569 NZ_AP022842:250473-250823 [Bacillus sp. KH172YL63]
TTGATTGTAAAGCGTGGTGACGTATATTTTGCAGACCTTTCTCCTGTTGTAGGTTCAGAACAAGGCGGAGTTCGGCCAGT
GCTTGTTATTCAAAACGATATAGGGAATAGGTTCAGTCCCACTATTATTGTAGCTGCCATTACCGCACAAATTCAAAAAG
CAAAACTGCCTACTCATGTTGAAATTGATGCAAAACGTTATGGCTTTGAACGTGATTCCGTTATCTTGCTAGAGCAGATT
CGAACGATTGATAAACAACGTCTGACCGATAAAATCACACACTTGGATGATGAGATGATGGAGAAGGTGGATGATGCCCT
GCAAATCAGTGTGGGTCTCATTCAATTCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10780.23 Da        Isoelectric Point: 5.9021

>AT36569 WP_032085355.1 NZ_AP022842:250187-250468 [Bacillus sp. KH172YL63]
VSESSATTEILIRLPQQLVTELDGFADQENVNRNEFIYRATKMYLRERKKRQLRESMRRGYMEMAKINLAIASEAIQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT36569 NZ_AP022842:250187-250468 [Bacillus sp. KH172YL63]
GTGTCTGAATCCAGCGCAACAACAGAAATCTTAATTCGTCTACCGCAACAGCTCGTTACAGAATTAGACGGCTTTGCAGA
TCAAGAAAATGTGAATCGCAATGAGTTTATTTATCGTGCAACCAAAATGTATTTAAGAGAGCGTAAAAAGAGACAATTAC
GTGAGTCTATGAGACGCGGCTATATGGAAATGGCTAAGATTAATCTCGCGATCGCTTCTGAAGCGATTCAAGCAGAATAT
GAGGCAGAACATACAGTCGAACGCTTAGTAAGCGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366ENG2

Antitoxin

Source ID Structure
AlphaFold DB A0A0V8H6B8

References