Detailed information of TA system TA036568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 28247..28818
• Replicon: plasmid pTUM19329-2
• Accession: NZ_AP022841
• Organism: Legionella sp. TUM19329

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HRS36_RS18490
• Protein ID: WP_173238596.1
• Coordinates: 28247..28504 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HRS36_RS18495
• Protein ID: WP_133137737.1
• Coordinates: 28498..28818 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS36_RS18480	24404..25327	+	924	WP_173238594.1	tyrosine-type recombinase/integrase	-
HRS36_RS18485	25311..28181	+	2871	WP_173238595.1	Tn3 family transposase	-
HRS36_RS18490	28247..28504	+	258	WP_173238596.1	type II toxin-antitoxin system HicA family toxin	Toxin
HRS36_RS18495	28498..28818	+	321	WP_133137737.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HRS36_RS18500	28918..29313	-	396	WP_173238716.1	hypothetical protein	-
HRS36_RS18505	29548..30105	+	558	WP_173238717.1	hypothetical protein	-
HRS36_RS18510	30165..30563	-	399	WP_173238718.1	hypothetical protein	-
HRS36_RS18515	30538..31965	+	1428	WP_173238719.1	hypothetical protein	-
HRS36_RS18520	32096..33103	-	1008	WP_173238720.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..35454	35454

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (52-102)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9395.07 Da
• Isoelectric Point: 10.8668

>T36568 WP_173238596.1 NZ_AP022841:28247-28504 [Legionella sp. TUM19329]
MKAKHKKVLHSIFETPVNASIIWKDIESLLVSLGAELSEGRGSRVRIALNGVRAVFHRPHPKKEADKGAVVSMRRLLKEA
GVEPC

Nucleotide

• Length: 258 bp

>T36568 NZ_AP022841:28247-28504 [Legionella sp. TUM19329]
ATGAAAGCTAAACATAAAAAAGTGCTGCACAGCATATTTGAAACGCCAGTGAATGCCAGCATTATCTGGAAGGATATCGA
GTCGTTGCTTGTTAGCTTGGGCGCTGAATTATCCGAGGGGCGCGGATCACGAGTACGTATAGCGCTGAATGGTGTTCGGG
CTGTTTTTCATCGACCTCATCCTAAAAAAGAGGCCGATAAGGGAGCAGTTGTTTCAATGAGGCGTTTACTGAAAGAAGCA
GGAGTAGAGCCATGTTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12145.64 Da
• Isoelectric Point: 5.1529

>AT36568 WP_133137737.1 NZ_AP022841:28498-28818 [Legionella sp. TUM19329]
MLNYKGYLGHVEFDDENELFVGEVINTKDVITFQADTAHGLKQAFVESIDDYIDFCHERNEKPEKPFSGKFNLRISPELH
REAYVAAKHSGMSLNSWVCDVLKHAV

Nucleotide

• Length: 321 bp

>AT36568 NZ_AP022841:28498-28818 [Legionella sp. TUM19329]
ATGTTAAATTATAAAGGTTATCTTGGTCATGTAGAGTTTGATGATGAAAATGAACTCTTTGTTGGCGAAGTCATTAATAC
CAAAGATGTCATTACGTTCCAGGCTGATACAGCGCATGGGCTAAAACAGGCATTTGTTGAGTCAATAGATGATTATATTG
ACTTTTGTCATGAGCGAAATGAAAAACCAGAAAAACCGTTTTCAGGGAAATTCAATCTGAGAATTTCTCCAGAGCTCCAT
CGTGAGGCCTATGTAGCAGCCAAACATTCTGGCATGAGCCTAAACTCATGGGTGTGTGATGTGTTGAAGCATGCTGTTTA
A