Detailed information of TA system TA036565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 49883..50345
• Replicon: plasmid pTUM19329-1
• Accession: NZ_AP022840
• Organism: Legionella sp. TUM19329

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: HRS36_RS17860
• Protein ID: WP_173238612.1
• Coordinates: 49883..50149 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: HRS36_RS17865
• Protein ID: WP_173238613.1
• Coordinates: 50133..50345 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS36_RS17840	45963..46391	+	429	WP_173238608.1	hypothetical protein	-
HRS36_RS17845	46412..46948	-	537	WP_173238609.1	DUF4879 domain-containing protein	-
HRS36_RS17850	47308..48207	+	900	WP_173238610.1	DNA polymerase III subunit epsilon	-
HRS36_RS17855	48807..49616	+	810	WP_173238611.1	hypothetical protein	-
HRS36_RS17860	49883..50149	-	267	WP_173238612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRS36_RS17865	50133..50345	-	213	WP_173238613.1	ribbon-helix-helix domain-containing protein	Antitoxin
HRS36_RS17870	50841..51977	-	1137	Protein_50	IS91 family transposase	-
HRS36_RS17875	51981..52835	-	855	WP_173238370.1	site-specific integrase	-
HRS36_RS17880	53047..54069	+	1023	WP_173238614.1	tyrosine-type recombinase/integrase	-
HRS36_RS17885	54193..54447	+	255	WP_173238615.1	type II toxin-antitoxin system ParD family antitoxin	-
HRS36_RS17890	54437..54733	+	297	WP_173238616.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..140878	140878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-38)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10298.98 Da
• Isoelectric Point: 10.0449

>T36565 WP_173238612.1 NZ_AP022840:c50149-49883 [Legionella sp. TUM19329]
MTWKIEFDADVEKDLKKLGHTAQKKILNYLKEKIASTDNPRLLGKSLSGNLSGLWRYRIGDYRIIAKIEDYHFIILVVHV
GHRKNVYD

Nucleotide

• Length: 267 bp

>T36565 NZ_AP022840:c50149-49883 [Legionella sp. TUM19329]
TTGACCTGGAAGATTGAGTTTGATGCTGATGTAGAAAAAGACCTTAAGAAACTTGGTCATACGGCACAGAAAAAAATTTT
GAACTATCTTAAAGAAAAGATCGCCTCGACTGATAATCCTCGCTTATTGGGGAAGTCCTTATCAGGCAACCTCTCTGGGT
TGTGGCGTTATCGTATTGGGGATTATCGTATTATTGCAAAAATTGAAGACTATCATTTTATAATTCTTGTCGTTCATGTA
GGGCATCGAAAAAATGTTTATGATTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8382.47 Da
• Isoelectric Point: 4.6882

>AT36565 WP_173238613.1 NZ_AP022840:c50345-50133 [Legionella sp. TUM19329]
MLAVRLPENLEERLSQLAERTHRSKSYYVKQALQDFLDEQEDHLIALSRLEKKNPRITLEEMESRLDLED

Nucleotide

• Length: 213 bp

>AT36565 NZ_AP022840:c50345-50133 [Legionella sp. TUM19329]
ATGTTAGCAGTTCGTTTACCAGAAAATCTTGAAGAGCGACTGTCTCAATTGGCAGAGCGAACGCATCGTTCTAAAAGTTA
TTATGTGAAACAAGCCTTACAAGATTTTCTCGATGAACAAGAAGATCATCTCATCGCCCTTTCTCGCCTGGAAAAGAAAA
ATCCTCGTATAACTCTTGAAGAAATGGAAAGCCGCCTTGACCTGGAAGATTGA