Detailed information of TA system TA036563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 26743..27314
• Replicon: plasmid pTUM19329-1
• Accession: NZ_AP022840
• Organism: Legionella sp. TUM19329

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HRS36_RS17755
• Protein ID: WP_173238596.1
• Coordinates: 27057..27314 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HRS36_RS17750
• Protein ID: WP_133137737.1
• Coordinates: 26743..27063 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS36_RS17735	22467..24017	-	1551	WP_173236263.1	IS21 family transposase	-
HRS36_RS17740	24533..26080	+	1548	WP_173235442.1	IS21 family transposase	-
HRS36_RS17745	26073..26639	+	567	WP_173238599.1	ATP-binding protein	-
HRS36_RS17750	26743..27063	-	321	WP_133137737.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HRS36_RS17755	27057..27314	-	258	WP_173238596.1	type II toxin-antitoxin system HicA family toxin	Toxin
HRS36_RS17760	27380..30250	-	2871	WP_173238595.1	Tn3 family transposase	-
HRS36_RS17765	30234..31157	-	924	WP_173238594.1	tyrosine-type recombinase/integrase	-
HRS36_RS17770	31416..31592	+	177	WP_173238103.1	ATP-binding protein	-
HRS36_RS17775	31643..31930	-	288	WP_173238600.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..140878	140878

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (52-102)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9395.07 Da
• Isoelectric Point: 10.8668

>T36563 WP_173238596.1 NZ_AP022840:c27314-27057 [Legionella sp. TUM19329]
MKAKHKKVLHSIFETPVNASIIWKDIESLLVSLGAELSEGRGSRVRIALNGVRAVFHRPHPKKEADKGAVVSMRRLLKEA
GVEPC

Nucleotide

• Length: 258 bp

>T36563 NZ_AP022840:c27314-27057 [Legionella sp. TUM19329]
ATGAAAGCTAAACATAAAAAAGTGCTGCACAGCATATTTGAAACGCCAGTGAATGCCAGCATTATCTGGAAGGATATCGA
GTCGTTGCTTGTTAGCTTGGGCGCTGAATTATCCGAGGGGCGCGGATCACGAGTACGTATAGCGCTGAATGGTGTTCGGG
CTGTTTTTCATCGACCTCATCCTAAAAAAGAGGCCGATAAGGGAGCAGTTGTTTCAATGAGGCGTTTACTGAAAGAAGCA
GGAGTAGAGCCATGTTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12145.64 Da
• Isoelectric Point: 5.1529

>AT36563 WP_133137737.1 NZ_AP022840:c27063-26743 [Legionella sp. TUM19329]
MLNYKGYLGHVEFDDENELFVGEVINTKDVITFQADTAHGLKQAFVESIDDYIDFCHERNEKPEKPFSGKFNLRISPELH
REAYVAAKHSGMSLNSWVCDVLKHAV

Nucleotide

• Length: 321 bp

>AT36563 NZ_AP022840:c27063-26743 [Legionella sp. TUM19329]
ATGTTAAATTATAAAGGTTATCTTGGTCATGTAGAGTTTGATGATGAAAATGAACTCTTTGTTGGCGAAGTCATTAATAC
CAAAGATGTCATTACGTTCCAGGCTGATACAGCGCATGGGCTAAAACAGGCATTTGTTGAGTCAATAGATGATTATATTG
ACTTTTGTCATGAGCGAAATGAAAAACCAGAAAAACCGTTTTCAGGGAAATTCAATCTGAGAATTTCTCCAGAGCTCCAT
CGTGAGGCCTATGTAGCAGCCAAACATTCTGGCATGAGCCTAAACTCATGGGTGTGTGATGTGTTGAAGCATGCTGTTTA
A