Detailed information of TA system TA036556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 887033..887592
• Replicon: chromosome
• Accession: NZ_AP022826
• Organism: Nitratiruptor labii strain HRV44

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: NIL_RS04535
• Protein ID: WP_187648416.1
• Coordinates: 887257..887592 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: NIL_RS04530
• Protein ID: WP_187648415.1
• Coordinates: 887033..887263 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NIL_RS04510	882609..882878	+	270	WP_187648412.1	acylphosphatase	-
NIL_RS04515	882899..884329	+	1431	WP_187648413.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
NIL_RS04520	884572..886437	+	1866	WP_197972137.1	TRAP transporter large permease subunit	-
NIL_RS04525	886447..886947	+	501	WP_187648414.1	HIT domain-containing protein	-
NIL_RS04530	887033..887263	+	231	WP_187648415.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NIL_RS04535	887257..887592	+	336	WP_187648416.1	endoribonuclease MazF	Toxin
NIL_RS04540	887593..887934	+	342	WP_187648417.1	nucleotide pyrophosphohydrolase	-
NIL_RS04545	887971..888744	+	774	WP_187648418.1	hypothetical protein	-
NIL_RS04550	888760..889950	-	1191	WP_187648419.1	hypothetical protein	-
NIL_RS04555	889999..890259	-	261	WP_187648420.1	hypothetical protein	-
NIL_RS04560	890316..891401	-	1086	WP_197972166.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
NIL_RS04565	891513..891818	+	306	WP_187648422.1	MTH1187 family thiamine-binding protein	-
NIL_RS04570	891822..892430	-	609	WP_187648423.1	class II aldolase/adducin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12512.68 Da
• Isoelectric Point: 9.0480

>T36556 WP_187648416.1 NZ_AP022826:887257-887592 [Nitratiruptor labii]
MVDYIPSKGDIVWINFNPQSGHEQSGKRPALVISPYKFNKITGFIICCPITSKIKDYPFEVVIEGKIKGAILSDQIKSLD
FRARGISFVEKAKNETLIEVIDKIKLILESQ

Nucleotide

• Length: 336 bp

>T36556 NZ_AP022826:887257-887592 [Nitratiruptor labii]
ATGGTAGATTATATACCTTCAAAAGGGGATATTGTTTGGATAAATTTCAATCCTCAAAGTGGGCATGAACAGTCTGGAAA
AAGACCGGCACTTGTCATATCTCCTTATAAATTCAATAAAATTACGGGATTTATAATATGTTGTCCAATTACAAGTAAGA
TTAAAGATTATCCTTTTGAAGTAGTGATTGAAGGTAAGATAAAAGGAGCAATACTTAGTGATCAGATCAAAAGTTTAGAT
TTTAGAGCAAGAGGCATAAGTTTTGTTGAAAAGGCAAAAAACGAGACTTTAATAGAAGTTATAGATAAAATAAAACTGAT
TTTAGAGAGTCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8749.41 Da
• Isoelectric Point: 10.5404

>AT36556 WP_187648415.1 NZ_AP022826:887033-887263 [Nitratiruptor labii]
MEAVIKKWGNSLGIRIPKRILDMLNLSENKKVEIKVVGGNIVIKPKKSLDELLSKIEEKNRHKEIDFGKPQGKELW

Nucleotide

• Length: 231 bp

>AT36556 NZ_AP022826:887033-887263 [Nitratiruptor labii]
ATGGAAGCGGTAATAAAAAAATGGGGCAACAGCTTAGGTATTAGAATCCCAAAAAGGATTTTGGATATGTTGAATTTGAG
TGAAAATAAAAAAGTTGAGATAAAAGTTGTTGGTGGAAATATTGTTATAAAGCCTAAAAAATCATTAGATGAACTTTTAA
GTAAAATTGAAGAAAAAAATCGCCATAAAGAGATAGATTTTGGAAAACCTCAAGGCAAAGAGTTATGGTAG