Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 108904..109429 Replicon plasmid JE86-ST05_p1
Accession NZ_AP022816
Organism Escherichia coli strain JE86-ST05

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JL003_RS26850 Protein ID WP_001159871.1
Coordinates 108904..109209 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JL003_RS26855 Protein ID WP_000813634.1
Coordinates 109211..109429 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL003_RS26820 104171..105441 + 1271 Protein_120 translesion error-prone DNA polymerase V subunit UmuC -
JL003_RS26825 105446..105838 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
JL003_RS26830 105843..106814 - 972 WP_001103694.1 hypothetical protein -
JL003_RS26835 107043..107687 + 645 WP_000633911.1 AAA family ATPase -
JL003_RS26840 107681..107956 + 276 WP_000239529.1 hypothetical protein -
JL003_RS26845 108067..108903 - 837 WP_011666452.1 site-specific integrase -
JL003_RS26850 108904..109209 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JL003_RS26855 109211..109429 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JL003_RS26860 109990..110241 + 252 WP_115189130.1 hypothetical protein -
JL003_RS26865 110241..110831 + 591 WP_011666449.1 hypothetical protein -
JL003_RS26870 110849..111196 - 348 WP_024234846.1 hypothetical protein -
JL003_RS26875 111370..111660 + 291 WP_011666447.1 hypothetical protein -
JL003_RS26880 111682..112167 - 486 Protein_132 hypothetical protein -
JL003_RS26885 112253..112596 - 344 Protein_133 transposase -
JL003_RS26890 112709..113770 - 1062 WP_011666443.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - afaC-I / aap/aspU 1..114953 114953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T36543 WP_001159871.1 NZ_AP022816:c109209-108904 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T36543 NZ_AP022816:c109209-108904 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT36543 WP_000813634.1 NZ_AP022816:c109429-109211 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT36543 NZ_AP022816:c109429-109211 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References