Detailed information of TA system TA036542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 45322..45857
• Replicon: plasmid JE86-ST05_p1
• Accession: NZ_AP022816
• Organism: Escherichia coli strain JE86-ST05

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: JL003_RS26440
• Protein ID: WP_000222766.1
• Coordinates: 45322..45609 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: JL003_RS26445
• Protein ID: WP_001132900.1
• Coordinates: 45606..45857 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL003_RS26410	40529..40822	+	294	Protein_38	transposase	-
JL003_RS26415	41416..41766	+	351	WP_011666513.1	dispersin Aap	-
JL003_RS26420	41889..42621	-	733	Protein_40	transposase zinc-binding domain-containing protein	-
JL003_RS26425	43021..43964	-	944	Protein_41	IS3 family transposase	-
JL003_RS26430	43966..44850	+	885	Protein_42	IS21 family transposase	-
JL003_RS26435	44847..45116	+	270	Protein_43	IS21-like element IS100kyp family helper ATPase IstB	-
JL003_RS26440	45322..45609	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL003_RS26445	45606..45857	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JL003_RS26450	45930..46121	-	192	WP_000829597.1	hypothetical protein	-
JL003_RS26455	46822..47679	-	858	WP_011666504.1	incFII family plasmid replication initiator RepA	-
JL003_RS26460	47672..47746	-	75	WP_001365705.1	RepA leader peptide Tap	-
JL003_RS26465	47971..48231	-	261	WP_000083819.1	replication regulatory protein RepA	-
JL003_RS26470	48471..49061	-	591	WP_000766812.1	DUF2726 domain-containing protein	-
JL003_RS26475	49099..49308	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
JL003_RS26480	49354..49809	-	456	WP_137519678.1	thermonuclease family protein	-
JL003_RS26485	49879..50313	+	435	WP_001309734.1	IS66 family insertion sequence hypothetical protein	-
JL003_RS26490	50310..50660	+	351	WP_000624688.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I / aap/aspU	1..114953	114953
	-	inside	IScluster/Tn	-	aap/aspU	36479..52304	15825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T36542 WP_000222766.1 NZ_AP022816:c45609-45322 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T36542 NZ_AP022816:c45609-45322 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT36542 WP_001132900.1 NZ_AP022816:c45857-45606 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT36542 NZ_AP022816:c45857-45606 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2