Detailed information of TA system TA036540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4907481..4908282
• Replicon: chromosome
• Accession: NZ_AP022815
• Organism: Escherichia coli strain JE86-ST05

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: JL003_RS23995
• Protein ID: WP_001094430.1
• Coordinates: 4907481..4907858 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JL003_RS24000
• Protein ID: WP_096855618.1
• Coordinates: 4907905..4908282 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL003_RS23975	4903573..4905111	-	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-
JL003_RS23980	4905860..4906702	-	843	WP_096855615.1	DUF4942 domain-containing protein	-
JL003_RS23985	4906787..4906984	-	198	WP_001374283.1	DUF957 domain-containing protein	-
JL003_RS23990	4906996..4907484	-	489	WP_000761666.1	hypothetical protein	-
JL003_RS23995	4907481..4907858	-	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
JL003_RS24000	4907905..4908282	-	378	WP_096855618.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JL003_RS24005	4908356..4908577	-	222	WP_096855616.1	DUF987 domain-containing protein	-
JL003_RS24010	4908646..4909122	-	477	WP_122997650.1	RadC family protein	-
JL003_RS24015	4909137..4909616	-	480	WP_032233447.1	antirestriction protein	-
JL003_RS24020	4909716..4909856	+	141	WP_000997937.1	hypothetical protein	-
JL003_RS24025	4909882..4910700	-	819	WP_001175155.1	DUF945 domain-containing protein	-
JL003_RS24030	4910790..4911023	-	234	WP_001278293.1	DUF905 family protein	-
JL003_RS24035	4911029..4911706	-	678	WP_001097302.1	hypothetical protein	-
JL003_RS24040	4911854..4912534	-	681	WP_096855617.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T36540 WP_001094430.1 NZ_AP022815:c4907858-4907481 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T36540 NZ_AP022815:c4907858-4907481 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13725.59 Da
• Isoelectric Point: 6.7380

>AT36540 WP_096855618.1 NZ_AP022815:c4908282-4907905 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT36540 NZ_AP022815:c4908282-4907905 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7