Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4064444..4065075 Replicon chromosome
Accession NZ_AP022815
Organism Escherichia coli strain JE86-ST05

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JL003_RS20190 Protein ID WP_001259385.1
Coordinates 4064800..4065075 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JL003_RS20185 Protein ID WP_000593555.1
Coordinates 4064444..4064803 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL003_RS20155 4059556..4060029 + 474 WP_001161650.1 PTS sugar transporter subunit IIA -
JL003_RS20160 4060026..4060307 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JL003_RS20165 4060384..4061742 + 1359 WP_021565455.1 PTS galactitol transporter subunit IIC -
JL003_RS20170 4061735..4063243 + 1509 WP_021565454.1 xylulose kinase -
JL003_RS20175 4063233..4063502 + 270 WP_000933061.1 HPr family phosphocarrier protein -
JL003_RS20180 4063534..4064394 + 861 WP_001131788.1 class II aldolase -
JL003_RS20185 4064444..4064803 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JL003_RS20190 4064800..4065075 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JL003_RS20195 4065134..4066258 - 1125 WP_021565453.1 ABC transporter permease -
JL003_RS20200 4066258..4068993 - 2736 WP_021565452.1 ribosome-associated ATPase/putative transporter RbbA -
JL003_RS20205 4068990..4070057 - 1068 WP_021565451.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4058785..4073896 15111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T36536 WP_001259385.1 NZ_AP022815:c4065075-4064800 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T36536 NZ_AP022815:c4065075-4064800 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT36536 WP_000593555.1 NZ_AP022815:c4064803-4064444 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT36536 NZ_AP022815:c4064803-4064444 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References