Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1867627..1868265 Replicon chromosome
Accession NZ_AP022815
Organism Escherichia coli strain JE86-ST05

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JL003_RS09590 Protein ID WP_000813794.1
Coordinates 1867627..1867803 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JL003_RS09595 Protein ID WP_001270286.1
Coordinates 1867849..1868265 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL003_RS09570 1863246..1864460 - 1215 WP_071830296.1 BenE family transporter YdcO -
JL003_RS09575 1864513..1865049 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JL003_RS09580 1865122..1867083 + 1962 WP_137519643.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JL003_RS09585 1867175..1867405 - 231 WP_000494244.1 YncJ family protein -
JL003_RS09590 1867627..1867803 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JL003_RS09595 1867849..1868265 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JL003_RS09600 1868344..1869753 + 1410 WP_137519644.1 PLP-dependent aminotransferase family protein -
JL003_RS09605 1869995..1871140 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JL003_RS09610 1871158..1872171 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JL003_RS09615 1872172..1873113 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1862058..1863206 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T36520 WP_000813794.1 NZ_AP022815:1867627-1867803 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T36520 NZ_AP022815:1867627-1867803 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT36520 WP_001270286.1 NZ_AP022815:1867849-1868265 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT36520 NZ_AP022815:1867849-1868265 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References