Detailed information of TA system TA036515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1778..2337
• Replicon: plasmid JE86-ST02_p2
• Accession: NZ_AP022813
• Organism: Escherichia coli strain JE86-ST02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: JLZ98_RS26760
• Protein ID: WP_000421257.1
• Coordinates: 2062..2337 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: JLZ98_RS26755
• Protein ID: WP_001178087.1
• Coordinates: 1778..2062 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ98_RS26750	1..879	+	879	WP_000729439.1	incFII family plasmid replication initiator RepA	-
JLZ98_RS26755	1778..2062	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLZ98_RS26760	2062..2337	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLZ98_RS26765	2443..2724	+	282	WP_001105066.1	hypothetical protein	-
JLZ98_RS26770	2915..3181	-	267	WP_000627831.1	hypothetical protein	-
JLZ98_RS26775	3319..3921	-	603	WP_000517694.1	hypothetical protein	-
JLZ98_RS26780	4275..5621	+	1347	WP_103927857.1	hypothetical protein	-
JLZ98_RS26785	6179..6481	-	303	WP_001245884.1	hypothetical protein	-
JLZ98_RS26790	6478..7104	-	627	WP_000587689.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83276	83276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T36515 WP_000421257.1 NZ_AP022813:2062-2337 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T36515 NZ_AP022813:2062-2337 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT36515 WP_001178087.1 NZ_AP022813:1778-2062 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT36515 NZ_AP022813:1778-2062 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCTCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625