Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 108852..109377 Replicon plasmid JE86-ST02_p1
Accession NZ_AP022812
Organism Escherichia coli strain JE86-ST02

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JLZ98_RS26705 Protein ID WP_001159871.1
Coordinates 108852..109157 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JLZ98_RS26710 Protein ID WP_000813634.1
Coordinates 109159..109377 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLZ98_RS26675 104119..105389 + 1271 Protein_120 translesion error-prone DNA polymerase V subunit UmuC -
JLZ98_RS26680 105394..105786 - 393 WP_000340833.1 plasmid partitioning/stability family protein -
JLZ98_RS26685 105791..106762 - 972 WP_001103694.1 hypothetical protein -
JLZ98_RS26690 106991..107635 + 645 WP_000633911.1 AAA family ATPase -
JLZ98_RS26695 107629..107904 + 276 WP_000239529.1 hypothetical protein -
JLZ98_RS26700 108015..108851 - 837 WP_011666452.1 site-specific integrase -
JLZ98_RS26705 108852..109157 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JLZ98_RS26710 109159..109377 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JLZ98_RS26715 109938..110189 + 252 WP_115189130.1 hypothetical protein -
JLZ98_RS26720 110189..110779 + 591 WP_011666449.1 hypothetical protein -
JLZ98_RS26725 110797..111144 - 348 WP_024234846.1 hypothetical protein -
JLZ98_RS26730 111318..111608 + 291 WP_011666447.1 hypothetical protein -
JLZ98_RS26735 111630..112115 - 486 Protein_132 hypothetical protein -
JLZ98_RS26740 112201..112544 - 344 Protein_133 transposase -
JLZ98_RS26745 112657..113718 - 1062 WP_011666443.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - afaC-I / aap/aspU 1..114901 114901


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T36514 WP_001159871.1 NZ_AP022812:c109157-108852 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T36514 NZ_AP022812:c109157-108852 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT36514 WP_000813634.1 NZ_AP022812:c109377-109159 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT36514 NZ_AP022812:c109377-109159 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References