Detailed information of TA system TA036513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 45327..45862
• Replicon: plasmid JE86-ST02_p1
• Accession: NZ_AP022812
• Organism: Escherichia coli strain JE86-ST02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: JLZ98_RS26295
• Protein ID: WP_000222766.1
• Coordinates: 45327..45614 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: JLZ98_RS26300
• Protein ID: WP_001132900.1
• Coordinates: 45611..45862 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ98_RS26265	40534..40827	+	294	Protein_38	transposase	-
JLZ98_RS26270	41421..41771	+	351	WP_011666513.1	dispersin Aap	-
JLZ98_RS26275	41894..42626	-	733	Protein_40	transposase zinc-binding domain-containing protein	-
JLZ98_RS26280	43026..43969	-	944	Protein_41	IS3 family transposase	-
JLZ98_RS26285	43971..44855	+	885	Protein_42	IS21 family transposase	-
JLZ98_RS26290	44852..45121	+	270	Protein_43	IS21-like element IS100kyp family helper ATPase IstB	-
JLZ98_RS26295	45327..45614	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLZ98_RS26300	45611..45862	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JLZ98_RS26305	45935..46126	-	192	WP_000829597.1	hypothetical protein	-
JLZ98_RS26310	46827..47684	-	858	WP_011666504.1	incFII family plasmid replication initiator RepA	-
JLZ98_RS26315	47677..47751	-	75	WP_001365705.1	RepA leader peptide Tap	-
JLZ98_RS26320	47976..48236	-	261	WP_000083819.1	replication regulatory protein RepA	-
JLZ98_RS26325	48476..49066	-	591	WP_000766812.1	DUF2726 domain-containing protein	-
JLZ98_RS26330	49104..49313	-	210	WP_001333231.1	hemolysin expression modulator Hha	-
JLZ98_RS26335	49359..49814	-	456	WP_137519678.1	thermonuclease family protein	-
JLZ98_RS26340	49884..50318	+	435	WP_001309734.1	IS66 family insertion sequence hypothetical protein	-
JLZ98_RS26345	50315..50665	+	351	WP_000624688.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I / aap/aspU	1..114901	114901
	-	inside	IScluster/Tn	-	aap/aspU	36483..52309	15826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T36513 WP_000222766.1 NZ_AP022812:c45614-45327 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T36513 NZ_AP022812:c45614-45327 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCGGCAAAACTAAGAGGGCTAAAGGACTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT36513 WP_001132900.1 NZ_AP022812:c45862-45611 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT36513 NZ_AP022812:c45862-45611 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2