Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4100191..4100822 Replicon chromosome
Accession NZ_AP022811
Organism Escherichia coli strain JE86-ST02

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JLZ98_RS20485 Protein ID WP_001259385.1
Coordinates 4100547..4100822 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JLZ98_RS20480 Protein ID WP_000593555.1
Coordinates 4100191..4100550 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLZ98_RS20450 4095303..4095776 + 474 WP_001161650.1 PTS sugar transporter subunit IIA -
JLZ98_RS20455 4095773..4096054 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JLZ98_RS20460 4096131..4097489 + 1359 WP_021565455.1 PTS galactitol transporter subunit IIC -
JLZ98_RS20465 4097482..4098990 + 1509 WP_021565454.1 xylulose kinase -
JLZ98_RS20470 4098980..4099249 + 270 WP_000933061.1 HPr family phosphocarrier protein -
JLZ98_RS20475 4099281..4100141 + 861 WP_001131788.1 class II aldolase -
JLZ98_RS20480 4100191..4100550 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JLZ98_RS20485 4100547..4100822 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JLZ98_RS20490 4100881..4102005 - 1125 WP_021565453.1 ABC transporter permease -
JLZ98_RS20495 4102005..4104740 - 2736 WP_021565452.1 ribosome-associated ATPase/putative transporter RbbA -
JLZ98_RS20500 4104737..4105804 - 1068 WP_021565451.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4094532..4109643 15111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T36507 WP_001259385.1 NZ_AP022811:c4100822-4100547 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T36507 NZ_AP022811:c4100822-4100547 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT36507 WP_000593555.1 NZ_AP022811:c4100550-4100191 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT36507 NZ_AP022811:c4100550-4100191 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References