Detailed information of TA system TA036503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3064638..3065263
• Replicon: chromosome
• Accession: NZ_AP022811
• Organism: Escherichia coli strain JE86-ST02

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLZ98_RS15395
• Protein ID: WP_000911330.1
• Coordinates: 3064638..3065036 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JLZ98_RS15400
• Protein ID: WP_000450524.1
• Coordinates: 3065036..3065263 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ98_RS15375	3060545..3060745	+	201	WP_000383836.1	YpfN family protein	-
JLZ98_RS15380	3060826..3061524	-	699	WP_000679812.1	esterase	-
JLZ98_RS15385	3061598..3063613	-	2016	WP_021565358.1	tRNA cytosine(34) acetyltransferase TmcA	-
JLZ98_RS15390	3063628..3064491	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
JLZ98_RS15395	3064638..3065036	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JLZ98_RS15400	3065036..3065263	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JLZ98_RS15405	3065417..3066130	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JLZ98_RS15410	3066343..3067377	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
JLZ98_RS15415	3067394..3068272	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JLZ98_RS15420	3068418..3068990	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JLZ98_RS15425	3068990..3069460	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T36503 WP_000911330.1 NZ_AP022811:c3065036-3064638 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T36503 NZ_AP022811:c3065036-3064638 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT36503 WP_000450524.1 NZ_AP022811:c3065263-3065036 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT36503 NZ_AP022811:c3065263-3065036 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33