Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1826181..1826819 Replicon chromosome
Accession NZ_AP022811
Organism Escherichia coli strain JE86-ST02

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JLZ98_RS09390 Protein ID WP_000813794.1
Coordinates 1826181..1826357 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JLZ98_RS09395 Protein ID WP_001270286.1
Coordinates 1826403..1826819 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLZ98_RS09370 1821800..1823014 - 1215 WP_071830296.1 BenE family transporter YdcO -
JLZ98_RS09375 1823067..1823603 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JLZ98_RS09380 1823676..1825637 + 1962 WP_137519643.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JLZ98_RS09385 1825729..1825959 - 231 WP_000494244.1 YncJ family protein -
JLZ98_RS09390 1826181..1826357 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JLZ98_RS09395 1826403..1826819 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JLZ98_RS09400 1826898..1828307 + 1410 WP_137519644.1 PLP-dependent aminotransferase family protein -
JLZ98_RS09405 1828549..1829694 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
JLZ98_RS09410 1829712..1830725 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JLZ98_RS09415 1830726..1831667 + 942 WP_001251319.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1820612..1821760 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T36491 WP_000813794.1 NZ_AP022811:1826181-1826357 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T36491 NZ_AP022811:1826181-1826357 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT36491 WP_001270286.1 NZ_AP022811:1826403-1826819 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT36491 NZ_AP022811:1826403-1826819 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References