Detailed information of TA system TA036483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 1867124..1867634
• Replicon: chromosome
• Accession: NZ_AP022673
• Organism: Sphingomonas sp. HMP9

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HMP09_RS08215
• Protein ID: WP_176499954.1
• Coordinates: 1867377..1867634 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: HMP09_RS08210
• Protein ID: WP_176501664.1
• Coordinates: 1867124..1867375 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMP09_RS08190	1862701..1863417	-	717	WP_176499950.1	7-cyano-7-deazaguanine synthase QueC	-
HMP09_RS08195	1863713..1864618	-	906	WP_176499951.1	Hsp33 family molecular chaperone HslO	-
HMP09_RS08200	1864767..1865687	-	921	WP_176499952.1	ornithine carbamoyltransferase	-
HMP09_RS08205	1865827..1867017	-	1191	WP_176499953.1	aspartate aminotransferase family protein	-
HMP09_RS08210	1867124..1867375	-	252	WP_176501664.1	BrnA antitoxin family protein	Antitoxin
HMP09_RS08215	1867377..1867634	-	258	WP_176499954.1	BrnT family toxin	Toxin
HMP09_RS08220	1867674..1869680	-	2007	WP_176499955.1	DNA topoisomerase IV subunit B	-
HMP09_RS08225	1869840..1870697	+	858	WP_176499956.1	outer membrane beta-barrel protein	-
HMP09_RS08230	1871412..1872182	-	771	WP_176499957.1	GcrA cell cycle regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1862065..1872182	10117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (9-81)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9893.27 Da
• Isoelectric Point: 9.4759

>T36483 WP_176499954.1 NZ_AP022673:c1867634-1867377 [Sphingomonas sp. HMP9]
VEFTFDPAKDAANIAKHGVSLAAFAGFDDDPIVRIDGRYEYGEVRYRAYGRIAGRGFCIVFMRREATIRLISFRRAHEKE
MRRYE

Nucleotide

• Length: 258 bp

>T36483 NZ_AP022673:c1867634-1867377 [Sphingomonas sp. HMP9]
GTGGAGTTTACGTTCGACCCTGCAAAAGACGCAGCCAACATCGCCAAGCATGGCGTGTCTCTGGCGGCGTTCGCAGGGTT
CGACGATGATCCGATCGTCAGGATCGACGGTCGATACGAATATGGGGAAGTTCGCTACCGTGCGTATGGCCGGATCGCTG
GCCGCGGTTTCTGTATCGTCTTCATGCGGAGAGAGGCGACGATCCGCCTCATCAGTTTTCGACGAGCGCATGAAAAGGAG
ATGCGGCGTTATGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9277.59 Da
• Isoelectric Point: 6.3352

>AT36483 WP_176501664.1 NZ_AP022673:c1867375-1867124 [Sphingomonas sp. HMP9]
MPPPPDFDENPEWTEEDFAKARPASEVLPPEVAALLVRRRGPQAAPTKKTVTLRLDPDVLDKFKATGPGWQSRMNEALRQ
AKV

Nucleotide

• Length: 252 bp

>AT36483 NZ_AP022673:c1867375-1867124 [Sphingomonas sp. HMP9]
ATGCCGCCACCGCCCGACTTCGACGAGAATCCCGAGTGGACCGAGGAGGATTTCGCCAAGGCCCGCCCCGCATCCGAAGT
CCTGCCCCCCGAAGTCGCCGCGCTGCTGGTACGCCGCCGCGGGCCGCAGGCCGCGCCGACCAAGAAGACGGTGACCCTCC
GCCTAGATCCGGACGTGCTCGACAAGTTCAAGGCCACCGGCCCGGGGTGGCAGTCGCGGATGAACGAGGCGCTGCGACAG
GCCAAGGTCTGA