Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 90097..90740 Replicon plasmid p1-09-02E
Accession NZ_AP022651
Organism Escherichia coli strain 09-02E

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag HRS14_RS25255 Protein ID WP_001034046.1
Coordinates 90324..90740 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag HRS14_RS25250 Protein ID WP_001261278.1
Coordinates 90097..90327 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS14_RS25220 85608..85913 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
HRS14_RS25225 85915..86133 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
HRS14_RS25230 86700..87212 + 513 WP_000151784.1 hypothetical protein -
HRS14_RS25235 87246..88379 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HRS14_RS25240 88546..89319 - 774 WP_000905949.1 hypothetical protein -
HRS14_RS25245 89332..89832 - 501 WP_001773886.1 hypothetical protein -
HRS14_RS25250 90097..90327 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HRS14_RS25255 90324..90740 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
HRS14_RS25260 90785..94579 - 3795 WP_001144732.1 hypothetical protein -
HRS14_RS25265 94960..95190 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HRS14_RS25270 95187..95603 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA5 / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..102029 102029


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T36479 WP_001034046.1 NZ_AP022651:90324-90740 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T36479 NZ_AP022651:90324-90740 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT36479 WP_001261278.1 NZ_AP022651:90097-90327 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT36479 NZ_AP022651:90097-90327 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References