Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 85608..86133 Replicon plasmid p1-09-02E
Accession NZ_AP022651
Organism Escherichia coli strain 09-02E

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HRS14_RS25220 Protein ID WP_001159868.1
Coordinates 85608..85913 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HRS14_RS25225 Protein ID WP_000813634.1
Coordinates 85915..86133 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS14_RS25205 81518..82684 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HRS14_RS25210 83272..84027 - 756 WP_000852146.1 replication initiation protein RepE -
HRS14_RS25215 84801..85607 - 807 WP_000016982.1 site-specific integrase -
HRS14_RS25220 85608..85913 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HRS14_RS25225 85915..86133 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HRS14_RS25230 86700..87212 + 513 WP_000151784.1 hypothetical protein -
HRS14_RS25235 87246..88379 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
HRS14_RS25240 88546..89319 - 774 WP_000905949.1 hypothetical protein -
HRS14_RS25245 89332..89832 - 501 WP_001773886.1 hypothetical protein -
HRS14_RS25250 90097..90327 + 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HRS14_RS25255 90324..90740 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA5 / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..102029 102029


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T36478 WP_001159868.1 NZ_AP022651:c85913-85608 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T36478 NZ_AP022651:c85913-85608 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT36478 WP_000813634.1 NZ_AP022651:c86133-85915 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT36478 NZ_AP022651:c86133-85915 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References