36452

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 526666..527284 Replicon chromosome
Accession NZ_AP022650
Organism Escherichia coli strain 09-02E

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HRS14_RS02455 Protein ID WP_001291435.1
Coordinates 526666..526884 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HRS14_RS02460 Protein ID WP_000344800.1
Coordinates 526910..527284 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS14_RS02420 521954..522526 + 573 WP_000779831.1 YbaY family lipoprotein -
HRS14_RS02425 522557..522868 - 312 WP_000409908.1 MGMT family protein -
HRS14_RS02435 523247..523600 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HRS14_RS02440 523642..525192 - 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HRS14_RS02445 525356..525826 - 471 WP_000136192.1 YlaC family protein -
HRS14_RS02450 525942..526493 - 552 WP_000102539.1 maltose O-acetyltransferase -
HRS14_RS02455 526666..526884 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HRS14_RS02460 526910..527284 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HRS14_RS02465 527830..530979 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
HRS14_RS02470 531002..532195 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T36452 WP_001291435.1 NZ_AP022650:c526884-526666 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T36452 NZ_AP022650:c526884-526666 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT36452 WP_000344800.1 NZ_AP022650:c527284-526910 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT36452 NZ_AP022650:c527284-526910 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References