Detailed information of TA system TA036447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4585527..4586666
• Replicon: chromosome
• Accession: NZ_AP022642
• Organism: Pseudomonas otitidis strain MrB4

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: PotMrB4_RS21100
• Protein ID: WP_107330689.1
• Coordinates: 4585527..4586276 (-)
• Length: 250 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1E4WFB3
• Locus tag: PotMrB4_RS21105
• Protein ID: WP_044405544.1
• Coordinates: 4586295..4586666 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PotMrB4_RS21090	4583198..4584559	-	1362	WP_160482899.1	DNA recombination protein RmuC	-
PotMrB4_RS21095	4584726..4585139	-	414	WP_044405541.1	sel1 repeat family protein	-
PotMrB4_RS21100	4585527..4586276	-	750	WP_107330689.1	RES family NAD+ phosphorylase	Toxin
PotMrB4_RS21105	4586295..4586666	-	372	WP_044405544.1	XRE family transcriptional regulator	Antitoxin
PotMrB4_RS21110	4586799..4587281	+	483	WP_172434404.1	GreA/GreB family elongation factor	-
PotMrB4_RS21115	4587318..4587713	+	396	WP_044405547.1	hypothetical protein	-
PotMrB4_RS21120	4587918..4588091	+	174	WP_172432200.1	hypothetical protein	-
PotMrB4_RS21125	4588119..4588544	-	426	WP_172434405.1	hypothetical protein	-
PotMrB4_RS21130	4588654..4590258	-	1605	WP_074971869.1	M28 family peptidase	-
PotMrB4_RS21135	4590534..4591199	-	666	WP_074971870.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (58-205)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 250 a.a.
• Molecular weight: 28161.05 Da
• Isoelectric Point: 6.9626

>T36447 WP_107330689.1 NZ_AP022642:c4586276-4585527 [Pseudomonas otitidis]
MDIWSACDGERHITPLRGRVVRLVESQEQVATLQLVDNLAEQALLEELIETSKPPLPRSAEPLHYLLKTPFRYPPLRWGS
RFGTVHEPSLFYAAARLETAMAEGAFYRFVLWSGMATPPPSGRILSEHASFEARFQVERGVSLQNPPFDAYPALLDRRDY
RATQALGAAMRAAGVEAFEYRSARCPQQGLNIALFTPDAFTERRPRNLTPWFCETTADYVAYKHAQAPDQPRLFRREAFL
VDGVIPQPA

Nucleotide

• Length: 750 bp

>T36447 NZ_AP022642:c4586276-4585527 [Pseudomonas otitidis]
ATGGACATCTGGAGCGCATGCGACGGCGAACGCCACATCACCCCTCTGCGCGGGCGCGTGGTGCGCCTGGTGGAGAGCCA
GGAACAGGTGGCGACCCTGCAACTGGTGGACAACCTCGCCGAACAGGCCCTGCTGGAAGAACTCATCGAGACCAGCAAAC
CGCCCCTGCCGCGCAGCGCCGAACCGCTGCACTACCTGCTCAAGACCCCGTTCCGCTACCCGCCGCTGCGCTGGGGCTCG
CGCTTCGGCACGGTGCACGAGCCCAGCCTGTTCTATGCCGCCGCGCGCCTGGAGACGGCCATGGCCGAAGGCGCCTTCTA
CCGCTTCGTGCTGTGGAGCGGCATGGCCACCCCACCGCCCAGCGGACGCATCCTCTCGGAGCACGCCTCCTTCGAGGCGC
GCTTCCAGGTGGAGCGCGGCGTGAGCCTGCAGAACCCGCCGTTCGATGCCTACCCGGCGCTGCTCGATCGCCGCGACTAC
CGCGCCACCCAGGCCCTGGGGGCGGCCATGCGTGCCGCCGGGGTCGAAGCCTTCGAATACCGCTCCGCCCGCTGCCCGCA
GCAGGGGCTGAACATCGCCCTGTTCACCCCCGACGCCTTCACCGAACGGCGCCCGCGCAACCTCACCCCCTGGTTCTGCG
AAACCACCGCCGACTACGTCGCCTACAAGCACGCCCAGGCGCCGGACCAGCCTCGTTTGTTCCGCCGCGAAGCCTTCCTG
GTGGACGGCGTCATCCCCCAGCCGGCGTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13690.98 Da
• Isoelectric Point: 10.6556

>AT36447 WP_044405544.1 NZ_AP022642:c4586666-4586295 [Pseudomonas otitidis]
MSALTRPHLDPDAVLAKALLNTREQLGLTRQQLADIVGVHRTVVTRWADAGSIRPQSKTGELALLVIRIYRALFALFGGN
LEDMRHFLRTENRHLGGVPLELMGQVQGLVRVVEYLDAIRGKV

Nucleotide

• Length: 372 bp

>AT36447 NZ_AP022642:c4586666-4586295 [Pseudomonas otitidis]
ATGAGCGCGCTGACCCGCCCCCACCTCGATCCCGACGCCGTCCTGGCCAAGGCCCTGCTCAACACCCGCGAGCAACTGGG
GCTGACCCGCCAGCAACTGGCCGACATCGTCGGCGTCCACCGCACCGTCGTCACCCGCTGGGCCGACGCCGGCAGCATCC
GCCCGCAGAGCAAGACCGGTGAACTGGCGCTGCTGGTGATCCGCATCTACCGCGCCCTGTTCGCGCTGTTCGGCGGCAAC
CTCGAGGACATGCGCCACTTCCTGCGCACCGAGAACCGTCACCTTGGCGGCGTACCGCTGGAGCTGATGGGGCAGGTGCA
GGGGCTGGTGCGGGTGGTGGAGTACCTCGACGCCATTCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E4WFB3