Detailed information of TA system TA036421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1776469..1777266
• Replicon: chromosome
• Accession: NZ_AP022628
• Organism: Enterobacter asburiae strain A2563

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HRR29_RS08555
• Protein ID: WP_172421424.1
• Coordinates: 1776766..1777266 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: HRR29_RS08550
• Protein ID: WP_008499829.1
• Coordinates: 1776469..1776738 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRR29_RS08520	1772096..1772539	-	444	WP_032658095.1	helix-turn-helix transcriptional regulator	-
HRR29_RS08525	1772692..1773933	+	1242	WP_033145159.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
HRR29_RS08530	1773967..1774194	-	228	WP_033145160.1	YccJ family protein	-
HRR29_RS08535	1774215..1774811	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
HRR29_RS08540	1775202..1775372	+	171	WP_023311077.1	general stress protein	-
HRR29_RS08545	1775490..1776407	+	918	WP_033145161.1	DMT family transporter	-
HRR29_RS08550	1776469..1776738	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
HRR29_RS08555	1776766..1777266	+	501	WP_172421424.1	GNAT family N-acetyltransferase	Toxin
HRR29_RS08560	1777340..1778662	-	1323	WP_033145163.1	pyrimidine utilization transport protein G	-
HRR29_RS08565	1778684..1779178	-	495	WP_032658088.1	pyrimidine utilization flavin reductase protein F	-
HRR29_RS08570	1779188..1779778	-	591	WP_033145164.1	malonic semialdehyde reductase	-
HRR29_RS08575	1779788..1780588	-	801	WP_033145165.1	pyrimidine utilization protein D	-
HRR29_RS08580	1780596..1780982	-	387	WP_033145166.1	pyrimidine utilization protein C	-
HRR29_RS08585	1780994..1781683	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18969.67 Da
• Isoelectric Point: 7.9145

>T36421 WP_172421424.1 NZ_AP022628:1776766-1777266 [Enterobacter asburiae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T36421 NZ_AP022628:1776766-1777266 [Enterobacter asburiae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGAGTCCTGGGTTATT
ACACTTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGAATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT36421 WP_008499829.1 NZ_AP022628:1776469-1776738 [Enterobacter asburiae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT36421 NZ_AP022628:1776469-1776738 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43