Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 591041..591617 Replicon chromosome
Accession NZ_AP022628
Organism Enterobacter asburiae strain A2563

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag HRR29_RS02740 Protein ID WP_010427219.1
Coordinates 591041..591328 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag HRR29_RS02745 Protein ID WP_033144548.1
Coordinates 591315..591617 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRR29_RS02715 586157..586852 + 696 WP_033144544.1 winged helix-turn-helix domain-containing protein -
HRR29_RS02720 587002..587472 + 471 WP_033144545.1 MarR family transcriptional regulator -
HRR29_RS02725 587469..588536 + 1068 WP_101744430.1 HlyD family secretion protein -
HRR29_RS02730 588526..589602 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
HRR29_RS02735 589599..590870 - 1272 WP_033144547.1 DUF445 domain-containing protein -
HRR29_RS02740 591041..591328 + 288 WP_010427219.1 BrnT family toxin Toxin
HRR29_RS02745 591315..591617 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
HRR29_RS02750 591648..592286 - 639 WP_033144549.1 LysE family translocator -
HRR29_RS02755 592334..593077 - 744 WP_033146481.1 AraC family transcriptional regulator -
HRR29_RS02760 593229..594599 + 1371 WP_033144550.1 NAD-dependent succinate-semialdehyde dehydrogenase -
HRR29_RS02765 594646..594966 - 321 WP_033144551.1 hypothetical protein -
HRR29_RS02770 594966..595511 - 546 WP_023310269.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T36418 WP_010427219.1 NZ_AP022628:591041-591328 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T36418 NZ_AP022628:591041-591328 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT36418 WP_033144548.1 NZ_AP022628:591315-591617 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT36418 NZ_AP022628:591315-591617 [Enterobacter asburiae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References