Detailed information of TA system TA036414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 44845..45530
• Replicon: plasmid pJCM30620_1
• Accession: NZ_AP022622
• Organism: Mycobacteroides abscessus strain JCM 30620

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4V0C7
• Locus tag: G6N22_RS25535
• Protein ID: WP_016341484.1
• Coordinates: 45120..45530 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N22_RS25530
• Protein ID: WP_016341485.1
• Coordinates: 44845..45123 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N22_RS25505	40780..41091	+	312	WP_041928357.1	hypothetical protein	-
G6N22_RS25510	41088..41396	+	309	WP_016341489.1	hypothetical protein	-
G6N22_RS25515	41393..43042	+	1650	WP_016341488.1	PPE protein	-
G6N22_RS25520	43039..43425	+	387	WP_016341487.1	hypothetical protein	-
G6N22_RS25525	43675..44655	+	981	WP_041928355.1	hypothetical protein	-
G6N22_RS25530	44845..45123	+	279	WP_016341485.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6N22_RS25535	45120..45530	+	411	WP_016341484.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N22_RS25540	45656..46618	+	963	WP_041928353.1	hypothetical protein	-
G6N22_RS25545	46687..47343	+	657	WP_051223733.1	hypothetical protein	-
G6N22_RS25550	47542..49857	+	2316	WP_131807328.1	hypothetical protein	-
G6N22_RS25555	49949..50293	+	345	WP_041928351.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119389	119389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-43)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14741.89 Da
• Isoelectric Point: 4.4809

>T36414 WP_016341484.1 NZ_AP022622:45120-45530 [Mycobacteroides abscessus]
VIYMDTSALTKLLIEEDETPELQAWLREQRGQDQYAATSAIGRVELIRVVARQGEPGLRERALYLLDGLDILPITAAVIT
LAERIGPATLRSLDAIHLASASQIAGELSAVVAYDIRLLEGCRAVGFRTQSPGAID

Nucleotide

• Length: 411 bp

>T36414 NZ_AP022622:45120-45530 [Mycobacteroides abscessus]
GTGATCTATATGGACACCTCAGCCCTGACGAAGCTGCTGATCGAGGAGGACGAGACACCGGAGCTGCAAGCCTGGCTCAG
AGAGCAACGAGGACAGGACCAGTACGCGGCGACCAGCGCCATCGGCCGAGTTGAGCTGATAAGAGTCGTTGCACGACAAG
GAGAGCCAGGCTTGCGTGAGCGGGCACTGTACTTGCTCGACGGGCTGGACATACTGCCGATCACCGCCGCCGTGATCACA
CTGGCCGAAAGGATCGGCCCGGCCACTCTCAGATCGTTGGATGCGATTCACCTTGCCTCGGCAAGCCAGATCGCCGGCGA
GCTATCAGCAGTCGTCGCCTACGACATCCGACTACTTGAGGGATGCCGGGCAGTCGGGTTCCGCACCCAATCCCCTGGCG
CAATCGACTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9941.38 Da
• Isoelectric Point: 8.7933

>AT36414 WP_016341485.1 NZ_AP022622:44845-45123 [Mycobacteroides abscessus]
MEMIGVRELRQNASPYLARVEAGETLGVTNNGRLIARLVPVGGAERSHEALISAGVLVPASRPNNLLDITATPRGSRKPS
LTTILHEMRDEQ

Nucleotide

• Length: 279 bp

>AT36414 NZ_AP022622:44845-45123 [Mycobacteroides abscessus]
ATGGAGATGATAGGCGTTCGGGAGCTGCGGCAGAACGCGTCACCGTATTTGGCCAGGGTGGAAGCGGGCGAGACCCTGGG
TGTAACGAACAACGGCAGGCTGATCGCGCGCCTTGTGCCTGTAGGGGGCGCAGAACGCTCCCATGAGGCATTGATCAGTG
CGGGCGTCCTGGTGCCCGCTAGTCGGCCAAACAACCTGCTCGATATCACAGCAACTCCCCGGGGGAGCCGGAAGCCGAGC
CTGACCACGATTCTTCACGAGATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: R4V0C7