Detailed information of TA system TA036413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 32648..33328
• Replicon: plasmid pJCM30620_1
• Accession: NZ_AP022622
• Organism: Mycobacteroides abscessus strain JCM 30620

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4UIW4
• Locus tag: G6N22_RS25450
• Protein ID: WP_016341421.1
• Coordinates: 32648..33052 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: R4V2I4
• Locus tag: G6N22_RS25455
• Protein ID: WP_016341420.1
• Coordinates: 33059..33328 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N22_RS25425	28762..29304	-	543	WP_041928277.1	hypothetical protein	-
G6N22_RS25430	29468..30157	+	690	WP_041928275.1	hypothetical protein	-
G6N22_RS25435	30177..31373	+	1197	WP_016341422.1	DUF1173 family protein	-
G6N22_RS25440	31370..31795	-	426	WP_131807335.1	hypothetical protein	-
G6N22_RS25445	32046..32225	-	180	WP_131807334.1	hypothetical protein	-
G6N22_RS25450	32648..33052	-	405	WP_016341421.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N22_RS25455	33059..33328	-	270	WP_016341420.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G6N22_RS25460	33418..34560	+	1143	WP_016341419.1	DUF4226 domain-containing protein	-
G6N22_RS25465	34633..35046	+	414	WP_131807333.1	hypothetical protein	-
G6N22_RS25470	35113..35376	+	264	WP_016341418.1	hypothetical protein	-
G6N22_RS25475	35379..36218	+	840	WP_016341417.1	hypothetical protein	-
G6N22_RS25480	36245..36754	+	510	WP_131807332.1	hypothetical protein	-
G6N22_RS25485	37016..37945	+	930	WP_079276906.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119389	119389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14471.63 Da
• Isoelectric Point: 6.0787

>T36413 WP_016341421.1 NZ_AP022622:c33052-32648 [Mycobacteroides abscessus]
VIYLDTSALVKLIRIEAESEALSEWLDERTHTPWITSVLAEVELPRAIRAAAPQGLAHVPLVLARFNRFEIDPVIRAAAA
AYPHRLRSLDAIHLATAQTAGAAVPLTAFVVYDQRLRQSAEDLGIITASPGAAA

Nucleotide

• Length: 405 bp

>T36413 NZ_AP022622:c33052-32648 [Mycobacteroides abscessus]
GTGATCTATCTGGACACCTCGGCGCTGGTCAAGCTCATCCGGATCGAGGCAGAATCCGAAGCGCTCTCCGAATGGTTGGA
CGAGCGCACGCACACTCCGTGGATAACCTCGGTACTGGCCGAAGTCGAGCTACCCCGGGCAATCCGCGCCGCCGCCCCGC
AAGGCCTGGCCCATGTACCCCTCGTGTTGGCGCGGTTCAACCGGTTCGAAATCGATCCCGTCATCCGTGCGGCAGCGGCC
GCCTACCCTCACCGGCTGCGATCCCTCGACGCCATACACCTGGCGACAGCCCAAACAGCCGGCGCTGCGGTGCCTTTGAC
CGCTTTCGTCGTCTACGACCAGCGCTTACGCCAATCTGCCGAAGACCTCGGCATCATCACTGCTAGCCCAGGTGCAGCGG
CCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9491.99 Da
• Isoelectric Point: 6.7252

>AT36413 WP_016341420.1 NZ_AP022622:c33328-33059 [Mycobacteroides abscessus]
MGEVPIRELNQQTRKVINRVKAGEEIAVTDHGELIAHIIPAATGPLAALISAGKVLPAAQGPAPRAMIHAPDQPEAGVLL
EKLRSEERY

Nucleotide

• Length: 270 bp

>AT36413 NZ_AP022622:c33328-33059 [Mycobacteroides abscessus]
ATGGGTGAAGTGCCAATACGTGAACTCAACCAACAGACCCGGAAAGTCATCAACCGTGTCAAGGCTGGCGAGGAAATCGC
CGTCACCGATCACGGCGAGCTCATCGCTCACATCATCCCGGCGGCCACCGGCCCTCTGGCCGCGTTGATCAGCGCGGGCA
AGGTCCTACCCGCGGCCCAGGGCCCGGCCCCGCGCGCCATGATCCATGCCCCCGATCAGCCCGAGGCTGGGGTATTGCTG
GAGAAGCTGCGCAGCGAGGAAAGGTATTAG

Structures

Toxin

• AlphaFold DB: R4UIW4

Antitoxin

• AlphaFold DB: R4V2I4