Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2850308..2850872 Replicon chromosome
Accession NZ_AP022618
Organism Mycolicibacterium insubricum strain JCM 16366

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag G6N16_RS13530 Protein ID WP_083032173.1
Coordinates 2850525..2850872 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag G6N16_RS13525 Protein ID WP_083032152.1
Coordinates 2850308..2850538 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6N16_RS13500 2846509..2847060 + 552 WP_083032145.1 YaeQ family protein -
G6N16_RS13505 2847177..2847620 - 444 WP_083032170.1 PIN domain-containing protein -
G6N16_RS13510 2847626..2847889 - 264 WP_133052969.1 hypothetical protein -
G6N16_RS13515 2848026..2849711 + 1686 WP_083032149.1 GMC family oxidoreductase N-terminal domain-containing protein -
G6N16_RS13520 2849716..2850222 - 507 WP_083032150.1 SRPBCC family protein -
G6N16_RS13525 2850308..2850538 + 231 WP_083032152.1 antitoxin Antitoxin
G6N16_RS13530 2850525..2850872 + 348 WP_083032173.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G6N16_RS13535 2850878..2851282 - 405 WP_179961257.1 cellulose-binding domain-containing protein -
G6N16_RS13540 2851447..2852079 - 633 WP_083032154.1 hypothetical protein -
G6N16_RS13545 2852296..2853225 + 930 WP_083032155.1 alpha/beta hydrolase -
G6N16_RS13550 2853254..2854012 - 759 Protein_2684 hypothetical protein -
G6N16_RS13555 2854023..2854193 - 171 WP_163787883.1 hypothetical protein -
G6N16_RS13560 2854298..2854903 + 606 WP_083032157.1 class I SAM-dependent methyltransferase -
G6N16_RS13565 2854797..2855576 + 780 WP_083032158.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2828631..2851186 22555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12586.41 Da        Isoelectric Point: 10.9541

>T36404 WP_083032173.1 NZ_AP022618:2850525-2850872 [Mycolicibacterium insubricum]
MLRGEIWRVDLDPVRGSEANKQRPAIIVSNDRANATATQLGRGVVTVVPVTSNTEKVYPFQFLISRGPSGLDVDSKAQAE
QVRSVAVQRLTRLIGRVSPAELRGLDDALRLHLSL

Download         Length: 348 bp

>T36404 NZ_AP022618:2850525-2850872 [Mycolicibacterium insubricum]
ATGCTCCGCGGTGAGATCTGGCGGGTTGATCTCGATCCAGTCCGCGGCAGCGAAGCGAACAAACAGCGTCCCGCAATTAT
CGTCAGCAATGACCGGGCCAATGCCACCGCGACCCAGTTGGGCCGGGGCGTGGTGACCGTCGTGCCTGTGACCAGCAACA
CCGAGAAGGTGTATCCCTTCCAGTTTTTGATCTCACGCGGACCCAGCGGTCTCGACGTCGACTCCAAGGCCCAGGCTGAG
CAAGTCAGATCGGTTGCGGTACAACGCCTGACCCGGCTGATCGGGCGCGTGTCCCCCGCAGAACTTCGCGGACTCGACGA
CGCCCTCAGACTTCACCTGTCGCTGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8320.09 Da        Isoelectric Point: 3.9934

>AT36404 WP_083032152.1 NZ_AP022618:2850308-2850538 [Mycolicibacterium insubricum]
MKLSVSLSDDDVSTLDAYVARIGLPSRSAGVQRAIQMLRHPDLEKDYAEAWGEWDAGEDSESWDNVIGDGLADAPR

Download         Length: 231 bp

>AT36404 NZ_AP022618:2850308-2850538 [Mycolicibacterium insubricum]
ATGAAACTCAGTGTCAGCCTTTCCGACGACGATGTTTCGACGTTGGATGCCTATGTCGCGCGCATCGGCCTGCCGTCCAG
GTCGGCGGGCGTACAAAGGGCGATCCAGATGCTGCGCCACCCGGACCTCGAAAAGGACTATGCCGAGGCGTGGGGCGAAT
GGGACGCCGGCGAGGACTCCGAATCCTGGGACAACGTCATCGGGGATGGACTCGCCGATGCTCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References