Detailed information of TA system TA036397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3015967..3016619
• Replicon: chromosome
• Accession: NZ_AP022616
• Organism: Mycolicibacterium phocaicum strain JCM 15301

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: A0A7I7ZQC2
• Locus tag: G6N46_RS14530
• Protein ID: WP_073697853.1
• Coordinates: 3015967..3016404 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A8H2JAZ7
• Locus tag: G6N46_RS14535
• Protein ID: WP_020098938.1
• Coordinates: 3016407..3016619 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N46_RS14510	3011250..3012929	+	1680	WP_138247953.1	NAD-binding protein	-
G6N46_RS14515	3012938..3013186	-	249	WP_073697855.1	hypothetical protein	-
G6N46_RS14520	3013294..3014064	-	771	WP_073697854.1	VOC family protein	-
G6N46_RS14525	3014280..3015866	-	1587	WP_138247952.1	transposase	-
G6N46_RS14530	3015967..3016404	-	438	WP_073697853.1	SRPBCC family protein	Toxin
G6N46_RS14535	3016407..3016619	-	213	WP_020098938.1	antitoxin	Antitoxin
G6N46_RS14540	3016669..3019077	-	2409	WP_138247951.1	HAD-IC family P-type ATPase	-
G6N46_RS14545	3019151..3020266	-	1116	WP_138247950.1	MBL fold metallo-hydrolase	-
G6N46_RS14550	3020266..3021000	-	735	WP_061006583.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15650.15 Da
• Isoelectric Point: 9.5938

>T36397 WP_073697853.1 NZ_AP022616:c3016404-3015967 [Mycolicibacterium phocaicum]
MAKLASSIEVPLSPEKAWEAASDLSRYDDWLSIHRMWRSPLPETLGKGTQISSVVEVKGMLNRVDWTIVHYNPPGSLTLN
GVGKGGVRVKLMAKITPAEAGSNVAFDIHLGGPALFGPIGMVVAAALRSDIHRSLDKFKELFASV

Nucleotide

• Length: 438 bp

>T36397 NZ_AP022616:c3016404-3015967 [Mycolicibacterium phocaicum]
ATGGCAAAACTCGCAAGTTCCATCGAAGTACCGCTGTCGCCCGAAAAGGCCTGGGAGGCAGCGTCGGACCTCTCCCGGTA
TGACGACTGGCTGTCCATCCACCGGATGTGGCGCAGCCCGTTGCCCGAGACCTTGGGGAAGGGCACGCAGATCTCGTCCG
TCGTCGAGGTCAAGGGCATGCTGAACCGGGTCGACTGGACCATCGTGCACTACAACCCGCCGGGATCGTTGACTCTCAAC
GGTGTCGGCAAGGGTGGGGTCCGGGTCAAGTTGATGGCCAAGATCACGCCGGCCGAAGCGGGTTCGAACGTGGCGTTCGA
CATCCACCTCGGCGGTCCGGCCCTGTTCGGGCCCATCGGCATGGTGGTGGCGGCCGCGCTGCGGTCCGACATCCACCGCT
CGCTGGACAAGTTCAAGGAGCTGTTCGCGTCGGTGTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7286.06 Da
• Isoelectric Point: 4.2092

>AT36397 WP_020098938.1 NZ_AP022616:c3016619-3016407 [Mycolicibacterium phocaicum]
MGFLDKVKDLAAQNADKVEAAIEKVGDIVDEKTEGKFKGVVDSAQEAAKKAVAGDQPAAEAPAEGTQEQQ

Nucleotide

• Length: 213 bp

>AT36397 NZ_AP022616:c3016619-3016407 [Mycolicibacterium phocaicum]
ATGGGATTTCTGGACAAGGTGAAGGACCTGGCGGCGCAGAACGCCGACAAGGTCGAGGCCGCTATCGAGAAGGTCGGCGA
CATCGTCGACGAGAAGACCGAAGGCAAGTTCAAGGGCGTTGTCGACAGCGCTCAGGAGGCGGCGAAGAAGGCCGTCGCAG
GGGACCAGCCTGCCGCTGAGGCTCCTGCCGAGGGGACGCAAGAACAGCAGTAG

Structures

Toxin

• AlphaFold DB: A0A7I7ZQC2