Detailed information of TA system TA036394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1315778..1316430
• Replicon: chromosome
• Accession: NZ_AP022616
• Organism: Mycolicibacterium phocaicum strain JCM 15301

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N46_RS06400
• Protein ID: WP_138248953.1
• Coordinates: 1315778..1316173 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7ZJC5
• Locus tag: G6N46_RS06405
• Protein ID: WP_064859434.1
• Coordinates: 1316170..1316430 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N46_RS06385	1311622..1313490	-	1869	WP_138248955.1	hypothetical protein	-
G6N46_RS06390	1313663..1315060	+	1398	WP_138248954.1	Fis family transcriptional regulator	-
G6N46_RS06395	1315173..1315610	+	438	WP_064859436.1	hypothetical protein	-
G6N46_RS06400	1315778..1316173	-	396	WP_138248953.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N46_RS06405	1316170..1316430	-	261	WP_064859434.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6N46_RS06410	1316470..1317666	-	1197	WP_138248952.1	winged helix-turn-helix domain-containing protein	-
G6N46_RS06415	1317807..1319840	+	2034	WP_138248951.1	DNA gyrase subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1306534..1324478	17944

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-39)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14398.73 Da
• Isoelectric Point: 6.8813

>T36394 WP_138248953.1 NZ_AP022616:c1316173-1315778 [Mycolicibacterium phocaicum]
MMLVDSDILIAHLRGIEVARDWLVSARRAGPLSISVVTVTELVGGMRSPERRAVWRLLDSMTVKPINELVARRAGDFMHE
HRRSNSGIGLADYLIAATAEINGLDLATLNIRHFPMFDGLEAPFALPAAPQ

Nucleotide

• Length: 396 bp

>T36394 NZ_AP022616:c1316173-1315778 [Mycolicibacterium phocaicum]
ATGATGCTTGTCGACTCCGACATCCTGATTGCCCATCTTCGCGGCATCGAGGTGGCACGCGACTGGTTGGTCAGTGCGCG
TCGTGCGGGACCATTGTCGATCAGCGTCGTCACGGTGACCGAACTCGTCGGCGGTATGCGCTCACCCGAACGCCGGGCTG
TGTGGCGGCTGCTCGATTCGATGACGGTCAAGCCCATCAACGAACTCGTCGCGCGACGCGCCGGAGACTTCATGCATGAA
CACCGTCGAAGCAACAGCGGTATCGGGCTTGCCGACTACCTGATCGCCGCGACAGCTGAGATCAACGGACTCGACCTCGC
GACCTTGAACATCAGGCACTTTCCGATGTTCGACGGACTGGAAGCGCCATTTGCGCTGCCGGCGGCGCCTCAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9615.70 Da
• Isoelectric Point: 4.1844

>AT36394 WP_064859434.1 NZ_AP022616:c1316430-1316170 [Mycolicibacterium phocaicum]
MKRTNIYLDEEQAALLDQLAAQEGLSRAELIRRMLDKALTGGGDSTLEADVAVLNDSFGVACDIAWPVRETGDREEHLEQ
VWNRTQ

Nucleotide

• Length: 261 bp

>AT36394 NZ_AP022616:c1316430-1316170 [Mycolicibacterium phocaicum]
ATGAAGCGCACCAACATCTACCTTGACGAGGAGCAGGCGGCGCTTCTCGACCAGCTGGCTGCGCAGGAGGGGCTATCCCG
GGCTGAACTGATTCGCCGCATGCTGGACAAGGCGTTGACCGGCGGTGGCGACAGCACGCTGGAAGCAGATGTCGCCGTGC
TCAACGACTCGTTCGGCGTGGCGTGCGATATCGCGTGGCCGGTGCGGGAAACGGGTGACCGCGAGGAGCATCTCGAGCAG
GTCTGGAACCGCACCCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7ZJC5