Detailed information of TA system TA036391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4998389..4999056
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N25_RS23185
• Protein ID: WP_083075166.1
• Coordinates: 4998389..4998820 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N25_RS23190
• Protein ID: WP_083075168.1
• Coordinates: 4998817..4999056 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS23165	4994592..4995827	-	1236	WP_083075161.1	hypothetical protein	-
G6N25_RS23170	4996452..4996831	-	380	Protein_4580	TetR/AcrR family transcriptional regulator	-
G6N25_RS23175	4997489..4997716	+	228	WP_083075162.1	antitoxin	-
G6N25_RS23180	4997713..4998102	+	390	WP_083075164.1	PIN domain-containing protein	-
G6N25_RS23185	4998389..4998820	-	432	WP_083075166.1	PIN domain-containing protein	Toxin
G6N25_RS23190	4998817..4999056	-	240	WP_083075168.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6N25_RS23195	4999567..4999797	+	231	WP_083075170.1	antitoxin	-
G6N25_RS23200	4999784..5000131	+	348	WP_083075171.1	type II toxin-antitoxin system PemK/MazF family toxin	-
G6N25_RS23205	5000339..5001148	-	810	WP_083075173.1	sulfotransferase	-
G6N25_RS23210	5001433..5001681	+	249	WP_083075175.1	hypothetical protein	-
G6N25_RS23215	5001766..5002959	-	1194	WP_083075176.1	PPE family protein	-
G6N25_RS23220	5002974..5003273	-	300	WP_083075178.1	PE family protein	-
G6N25_RS23225	5003502..5003696	+	195	WP_142272736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4972893..5016520	43627
	-	flank	IS/Tn	-	-	4992700..4994289	1589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15934.05 Da
• Isoelectric Point: 6.2101

>T36391 WP_083075166.1 NZ_AP022615:c4998820-4998389 [Mycobacterium heidelbergense]
VTRALLDVNVLIALLDSDHVDHERVRGWIDAEIVHGWASCAITQNGFARIVSQPRYPSPVPPAQAIARLARAAATEYHEY
WTCSVSLLDDELFDYTRLHGHRQVTDAYLLALATANGCRFATLDQTIPIDAVRRADARNLTLI

Nucleotide

• Length: 432 bp

>T36391 NZ_AP022615:c4998820-4998389 [Mycobacterium heidelbergense]
GTGACGCGCGCCCTGCTGGACGTCAATGTCCTCATTGCGCTGCTTGACAGCGATCACGTCGATCACGAGCGTGTTCGTGG
GTGGATCGACGCCGAGATCGTCCACGGCTGGGCGTCGTGTGCGATAACTCAGAACGGCTTCGCTCGGATCGTCAGCCAGC
CTCGATACCCCAGCCCGGTGCCACCTGCACAGGCAATCGCCAGGTTGGCTCGCGCCGCGGCGACCGAGTATCACGAGTAC
TGGACATGTTCGGTCAGTCTGCTCGACGACGAGCTTTTCGACTACACGCGGCTGCACGGACATCGTCAGGTGACCGACGC
ATATCTACTCGCGCTCGCAACCGCCAACGGCTGCCGGTTCGCGACTCTGGATCAGACGATTCCCATCGACGCTGTCCGGC
GCGCCGATGCGCGCAACCTGACGCTTATCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8893.88 Da
• Isoelectric Point: 4.8096

>AT36391 WP_083075168.1 NZ_AP022615:c4999056-4998817 [Mycobacterium heidelbergense]
MRTTLSLDDDVLLAVKERARRENRTAGEVLSDLARQALTQQCQTGTPTRESFYGFEPFEHRGAAVSNALIDRLRDEEAV

Nucleotide

• Length: 240 bp

>AT36391 NZ_AP022615:c4999056-4998817 [Mycobacterium heidelbergense]
ATGCGCACCACGCTGTCCTTGGATGATGACGTATTGCTCGCGGTGAAGGAACGCGCGCGCCGCGAGAACCGCACAGCAGG
CGAAGTTCTGTCCGATCTCGCGCGGCAGGCGCTGACACAACAGTGCCAGACCGGCACGCCCACACGGGAAAGCTTCTACG
GTTTCGAACCCTTCGAGCATCGAGGGGCTGCCGTGTCCAACGCTCTCATCGACCGGCTGCGTGACGAGGAAGCCGTGTGA