Detailed information of TA system TA036388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4352216..4352751
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N25_RS20325
• Protein ID: WP_083072497.1
• Coordinates: 4352216..4352548 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N25_RS20330
• Protein ID: WP_083072498.1
• Coordinates: 4352545..4352751 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS20305	4347512..4348489	-	978	WP_083072494.1	XRE family transcriptional regulator	-
G6N25_RS20310	4348458..4349039	-	582	WP_163672511.1	hypothetical protein	-
G6N25_RS20315	4349133..4350218	-	1086	WP_169924606.1	restriction endonuclease	-
G6N25_RS20320	4350659..4351984	+	1326	WP_142272473.1	hypothetical protein	-
G6N25_RS20325	4352216..4352548	-	333	WP_083072497.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N25_RS20330	4352545..4352751	-	207	WP_083072498.1	DUF3018 family protein	Antitoxin
G6N25_RS20335	4353016..4353771	+	756	WP_083072499.1	alpha/beta hydrolase	-
G6N25_RS20340	4353854..4354246	+	393	WP_083072584.1	MarR family transcriptional regulator	-
G6N25_RS20345	4354358..4354567	+	210	Protein_4019	saccharopine dehydrogenase	-
G6N25_RS20350	4354847..4355194	-	348	WP_083072500.1	hypothetical protein	-
G6N25_RS20355	4355197..4355655	-	459	WP_083072501.1	prevent-host-death protein	-
G6N25_RS20360	4355967..4356278	-	312	WP_083072502.1	type II toxin-antitoxin system PemK/MazF family toxin	-
G6N25_RS20365	4356268..4356501	-	234	WP_071510754.1	hypothetical protein	-
G6N25_RS20370	4356783..4357196	-	414	WP_083072585.1	type II toxin-antitoxin system VapC family toxin	-
G6N25_RS20375	4357217..4357453	-	237	WP_083072503.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4338273..4374800	36527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11938.83 Da
• Isoelectric Point: 10.0757

>T36388 WP_083072497.1 NZ_AP022615:c4352548-4352216 [Mycobacterium heidelbergense]
VNRGEIWTVAGGVYATKPRPAVIVQDDLFDATSSVTVAPMSRTLLDAPLMRIRIAGGHGRLSGLDHDSDVMIDKLTTIKR
SNVHARVGRLTAEQVVEVERAMMAFLGLAR

Nucleotide

• Length: 333 bp

>T36388 NZ_AP022615:c4352548-4352216 [Mycobacterium heidelbergense]
GTGAATAGAGGGGAGATCTGGACCGTAGCAGGGGGCGTCTACGCGACCAAACCGCGTCCCGCGGTGATTGTTCAGGATGA
CCTCTTTGATGCCACGAGTTCCGTAACTGTTGCTCCCATGTCCAGGACGCTGTTGGATGCGCCGTTGATGCGCATCCGGA
TAGCCGGCGGACACGGCCGGTTATCCGGACTTGATCACGACAGTGACGTGATGATCGACAAGCTCACAACCATCAAAAGG
TCAAACGTCCACGCCCGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGGTCGAACGAGCGATGATGGCATTCCTCGG
CCTTGCACGATAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7988.83 Da
• Isoelectric Point: 4.6412

>AT36388 WP_083072498.1 NZ_AP022615:c4352751-4352545 [Mycobacterium heidelbergense]
MAVRERVGEYRRRMRERGLRPLQVWVPDVRTESFAAEAHRQASLVARADESTDDQDFIEAISTPWDEE

Nucleotide

• Length: 207 bp

>AT36388 NZ_AP022615:c4352751-4352545 [Mycobacterium heidelbergense]
ATGGCGGTGAGGGAAAGAGTCGGCGAGTACCGACGGCGGATGCGGGAGCGGGGCCTGCGGCCGCTGCAGGTCTGGGTGCC
TGACGTGCGCACTGAGAGTTTTGCCGCCGAGGCACATCGTCAGGCTTCGTTGGTCGCACGAGCGGACGAAAGCACCGATG
ACCAGGACTTCATTGAGGCCATCTCGACGCCATGGGACGAGGAGTGA