Detailed information of TA system TA036386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 4004178..4004839
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N25_RS18710
• Protein ID: WP_083074431.1
• Coordinates: 4004444..4004839 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N25_RS18705
• Protein ID: WP_158084882.1
• Coordinates: 4004178..4004447 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS18685	3999355..4000947	-	1593	WP_083074436.1	Na+/H+ antiporter	-
G6N25_RS18690	4001261..4001695	+	435	WP_083074435.1	protease inhibitor I42 family protein	-
G6N25_RS18695	4001709..4002407	-	699	WP_083074434.1	alpha/beta hydrolase	-
G6N25_RS18700	4002601..4003986	-	1386	WP_083074433.1	FAD-binding oxidoreductase	-
G6N25_RS18705	4004178..4004447	+	270	WP_158084882.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G6N25_RS18710	4004444..4004839	+	396	WP_083074431.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N25_RS18715	4005125..4005421	+	297	WP_197745682.1	toxin-antitoxin system HicB family antitoxin	-
G6N25_RS18720	4005418..4005852	+	435	WP_083074429.1	type II toxin-antitoxin system VapC family toxin	-
G6N25_RS18725	4005971..4006258	+	288	Protein_3699	VOC family protein	-
G6N25_RS18730	4006404..4007084	-	681	WP_083074476.1	cysteine hydrolase	-
G6N25_RS23625	4007487..4007573	+	87	Protein_3701	enoyl-CoA hydratase/isomerase family protein	-
G6N25_RS18740	4007654..4008022	-	369	WP_083074428.1	ClpX C4-type zinc finger protein	-
G6N25_RS18745	4008223..4008990	+	768	WP_083074427.1	Rv3717 family N-acetylmuramoyl-L-alanine amidase	-
G6N25_RS18750	4009054..4009353	-	300	WP_083074475.1	DUF202 domain-containing protein	-
G6N25_RS18755	4009362..4009700	-	339	WP_142272664.1	DUF202 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-41)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 13879.86 Da
• Isoelectric Point: 6.2128

>T36386 WP_083074431.1 NZ_AP022615:4004444-4004839 [Mycobacterium heidelbergense]
MIYIDTSAAVKLVAAEQESAALIDWLNAHPDENLATSTVGHIELTRAAARAGPSAVALARDVASTIDTLILTEAIASASA
TIAPPELRTLDAIHLATAHIHRKALTAFCAYDRRLLEAAESHRLPIVSPRP

Nucleotide

• Length: 396 bp

>T36386 NZ_AP022615:4004444-4004839 [Mycobacterium heidelbergense]
ATGATTTACATCGATACGTCCGCCGCAGTGAAACTCGTGGCGGCTGAACAGGAGTCAGCGGCTCTGATCGATTGGCTCAA
TGCACACCCTGACGAGAACCTCGCCACCAGCACCGTCGGTCATATTGAGCTGACACGGGCGGCCGCACGAGCGGGACCCA
GCGCCGTCGCGCTCGCACGCGATGTCGCCTCGACGATCGACACCCTCATCTTGACCGAAGCCATCGCCTCGGCGTCCGCC
ACGATCGCGCCTCCCGAATTGCGCACCCTCGATGCGATACACCTCGCCACCGCACACATCCATCGAAAGGCCCTTACCGC
GTTCTGTGCCTACGATCGCCGGCTGCTCGAGGCGGCCGAATCGCACAGGCTACCGATCGTTTCTCCGCGGCCGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9622.11 Da
• Isoelectric Point: 6.2819

>AT36386 WP_158084882.1 NZ_AP022615:4004178-4004447 [Mycobacterium heidelbergense]
VDTIGIRELRQNASVWIAKVKAGLTIQITDRGLPVARLVPIMPAERARDQLIADGQLIPSSAPRVPLSTADLVEGPSLTA
ILDEQRSDR

Nucleotide

• Length: 270 bp

>AT36386 NZ_AP022615:4004178-4004447 [Mycobacterium heidelbergense]
GTGGACACAATCGGAATTCGTGAGCTACGCCAGAACGCGAGCGTGTGGATCGCGAAGGTAAAGGCGGGATTAACCATCCA
AATCACCGACCGCGGCCTGCCCGTGGCGCGGTTGGTACCGATCATGCCCGCCGAACGCGCCCGTGACCAACTCATCGCCG
ACGGCCAGCTGATCCCATCAAGCGCACCGCGAGTCCCGCTAAGTACGGCCGATCTCGTCGAAGGTCCGTCGCTCACCGCG
ATACTGGACGAACAACGCTCCGACCGATGA