Detailed information of TA system TA036383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2266260..2266886
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1X0DBA4
• Locus tag: G6N25_RS10615
• Protein ID: WP_071512907.1
• Coordinates: 2266260..2266658 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N25_RS10620
• Protein ID: WP_083076902.1
• Coordinates: 2266659..2266886 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS10600	2262350..2263816	+	1467	WP_083076893.1	glutamate synthase subunit beta	-
G6N25_RS10605	2264252..2265106	-	855	WP_083076896.1	LLM class F420-dependent oxidoreductase	-
G6N25_RS10610	2265200..2266207	+	1008	WP_083076899.1	PHP domain-containing protein	-
G6N25_RS10615	2266260..2266658	-	399	WP_071512907.1	PIN domain nuclease	Toxin
G6N25_RS10620	2266659..2266886	-	228	WP_083076902.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N25_RS10625	2266948..2268153	-	1206	WP_083076930.1	DNA polymerase IV	-
G6N25_RS10630	2268370..2269011	-	642	WP_083076905.1	TetR/AcrR family transcriptional regulator	-
G6N25_RS10635	2269088..2270557	+	1470	WP_083076908.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-119)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14977.28 Da
• Isoelectric Point: 7.2178

>T36383 WP_071512907.1 NZ_AP022615:c2266658-2266260 [Mycobacterium heidelbergense]
MIVDTSVWIEFLTTSESLASRWLADRIAANSQVIVPEVVMMELLIGTTDESKAIRRRRLLQRFNIEPFAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVTVAHRDRDYEVIRTHCGLQTEPLF

Nucleotide

• Length: 399 bp

>T36383 NZ_AP022615:c2266658-2266260 [Mycobacterium heidelbergense]
ATGATCGTCGACACGTCGGTCTGGATTGAATTTCTGACCACCTCTGAGTCGCTCGCCAGCCGCTGGCTAGCAGATCGCAT
TGCCGCCAACTCGCAGGTGATCGTCCCTGAGGTCGTGATGATGGAACTGCTGATCGGTACGACCGACGAGTCCAAGGCAA
TACGGCGGAGGCGACTACTGCAACGGTTCAATATCGAGCCGTTTGCCCCCGTGCGGGACGCAGAAGATGCGGCCGCTATC
CACCGGCGCTGTCGTCGCGGTGGTGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGCTGCGGATCGG
GGTCACTGTGGCACATCGTGATCGTGATTACGAGGTGATCCGCACGCATTGCGGACTACAAACCGAGCCCTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8276.13 Da
• Isoelectric Point: 4.0684

>AT36383 WP_083076902.1 NZ_AP022615:c2266886-2266659 [Mycobacterium heidelbergense]
MSRTNIEINDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLDRDEALALQGSGFDFTNDEIESFSDTGIGLADQS

Nucleotide

• Length: 228 bp

>AT36383 NZ_AP022615:c2266886-2266659 [Mycobacterium heidelbergense]
ATGTCGCGGACCAACATCGAAATCAACGACGAACTTGTGGCCGCCGCGCAGCGAATGTATCGACTGGATTCGAAGCGGAG
TGCCGTTGACCTCGCACTGCGCAGGCTCGTGGGTGAACCGTTAGACCGCGATGAGGCGTTGGCACTCCAGGGCAGCGGTT
TCGATTTCACCAATGATGAGATCGAATCGTTTTCGGACACGGGCATAGGCCTCGCAGACCAGTCTTAG

Structures

Toxin

• AlphaFold DB: A0A1X0DBA4