Detailed information of TA system TA036379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 1296700..1297346
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N25_RS06195
• Protein ID: WP_083074035.1
• Coordinates: 1296700..1297092 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N25_RS06200
• Protein ID: WP_083074012.1
• Coordinates: 1297089..1297346 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS06180	1293201..1294322	-	1122	WP_142272621.1	hypothetical protein	-
G6N25_RS06185	1295743..1296138	+	396	WP_083074010.1	DUF1778 domain-containing protein	-
G6N25_RS06190	1296135..1296713	+	579	WP_083074011.1	GNAT family N-acetyltransferase	-
G6N25_RS06195	1296700..1297092	-	393	WP_083074035.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N25_RS06200	1297089..1297346	-	258	WP_083074012.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6N25_RS06205	1297825..1298766	-	942	WP_083074013.1	class I SAM-dependent methyltransferase	-
G6N25_RS06210	1298769..1299710	-	942	WP_083074036.1	class I SAM-dependent methyltransferase	-
G6N25_RS06215	1299866..1302238	+	2373	WP_083074014.1	flippase-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-50)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14104.01 Da
• Isoelectric Point: 4.4978

>T36379 WP_083074035.1 NZ_AP022615:c1297092-1296700 [Mycobacterium heidelbergense]
VTVRRALADTSIFIGIEAARFDDDRFTEYEWGVSVITLGELRLGVLQASGPDTAARRLSTYQLAQRFEPLSIDETVSEAW
ALLVSKLRAANLRAPINDSWIAATAVAHGIPIVTQGSDYSAMPGVEVITI

Nucleotide

• Length: 393 bp

>T36379 NZ_AP022615:c1297092-1296700 [Mycobacterium heidelbergense]
GTGACGGTTCGGCGGGCCCTCGCCGACACGTCCATCTTCATCGGGATCGAGGCGGCACGATTCGACGACGACCGATTTAC
CGAATACGAATGGGGCGTCTCGGTGATCACACTGGGTGAGCTGCGGCTGGGTGTCCTGCAAGCCAGTGGCCCGGACACCG
CCGCACGCCGGCTCTCCACATACCAGCTTGCTCAGCGGTTCGAACCACTGAGCATCGACGAGACGGTCTCCGAAGCGTGG
GCATTGCTGGTATCCAAGCTGCGCGCCGCCAACCTGCGAGCACCGATCAACGACAGTTGGATTGCGGCAACTGCCGTGGC
GCATGGCATTCCGATCGTGACCCAGGGCAGCGACTACTCTGCGATGCCAGGCGTTGAGGTCATAACAATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9590.95 Da
• Isoelectric Point: 4.8279

>AT36379 WP_083074012.1 NZ_AP022615:c1297346-1297089 [Mycobacterium heidelbergense]
MAATIPARDLRNRTAEVLRRVAAGEEIEVLKDNRPVARIVPLTRRRQWLPAAEVIGELMRLGPDTTNLSEELPETLTQTT
DDVSW

Nucleotide

• Length: 258 bp

>AT36379 NZ_AP022615:c1297346-1297089 [Mycobacterium heidelbergense]
ATGGCTGCTACGATACCCGCCCGCGATCTCCGTAACCGCACCGCTGAGGTGCTGCGGCGAGTTGCGGCAGGCGAGGAGAT
CGAGGTGCTCAAGGACAATCGTCCTGTGGCGCGCATAGTCCCACTCACACGGCGCCGCCAATGGCTACCGGCAGCGGAGG
TAATCGGCGAACTCATGCGCCTGGGTCCCGATACCACCAACCTCAGCGAGGAACTGCCGGAGACGCTGACACAGACCACG
GACGACGTGTCGTGGTGA