Detailed information of TA system TA036375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 832523..833112
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: G6N25_RS03950
• Protein ID: WP_083073807.1
• Coordinates: 832523..832855 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: G6N25_RS03955
• Protein ID: WP_083073806.1
• Coordinates: 832855..833112 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS03930	827939..828754	-	816	WP_179961699.1	FAD/NAD(P)-binding protein	-
G6N25_RS03935	828765..829889	-	1125	WP_083073907.1	4Fe-4S dicluster domain-containing protein	-
G6N25_RS03940	829940..830932	-	993	WP_083073809.1	NAD(P)H nitroreductase	-
G6N25_RS03945	831050..832225	-	1176	WP_083073808.1	cation:proton antiporter	-
G6N25_RS03950	832523..832855	-	333	WP_083073807.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N25_RS03955	832855..833112	-	258	WP_083073806.1	antitoxin	Antitoxin
G6N25_RS03960	833287..833538	+	252	WP_083073805.1	helix-turn-helix domain-containing protein	-
G6N25_RS03965	833535..834905	+	1371	WP_083073804.1	HipA domain-containing protein	-
G6N25_RS03970	834987..835304	+	318	WP_083073803.1	hypothetical protein	-
G6N25_RS03975	835301..836638	+	1338	WP_083073802.1	helicase-associated domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11750.50 Da
• Isoelectric Point: 10.7596

>T36375 WP_083073807.1 NZ_AP022615:c832855-832523 [Mycobacterium heidelbergense]
IERGEIYIVSLDPTSGHEQRGTRPVLIVSPAAFNRLTKTPVVLPITRGGNFARTAGFAVSLADAGTRTTGVVRCDQPRAI
DIGSRDGRRVERVPPAVLGEVLAKLATVFE

Nucleotide

• Length: 333 bp

>T36375 NZ_AP022615:c832855-832523 [Mycobacterium heidelbergense]
ATTGAGCGCGGCGAAATCTACATTGTTTCGCTTGATCCAACATCGGGCCATGAGCAGCGTGGCACGCGCCCGGTGTTGAT
CGTGTCGCCAGCCGCATTTAATCGTCTGACGAAAACGCCGGTGGTGCTACCGATAACGCGCGGCGGAAACTTTGCCCGTA
CAGCGGGCTTCGCGGTATCGCTGGCAGATGCGGGCACCCGCACGACCGGCGTGGTTCGCTGCGATCAGCCACGCGCAATC
GATATTGGCTCGCGTGACGGCCGCAGAGTCGAACGTGTGCCGCCCGCGGTCTTGGGCGAAGTACTGGCCAAGCTTGCCAC
TGTCTTTGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9141.51 Da
• Isoelectric Point: 4.3707

>AT36375 WP_083073806.1 NZ_AP022615:c833112-832855 [Mycobacterium heidelbergense]
VYTTKLRKVGGSVMLAIPPAFLDVLRLHAGAAVALSVDEGQLVVEPQVRPRYTLDELLAQCDASADPTVEDREWLDGKPV
GGELL

Nucleotide

• Length: 258 bp

>AT36375 NZ_AP022615:c833112-832855 [Mycobacterium heidelbergense]
GTGTACACAACGAAGCTTCGCAAAGTCGGCGGTTCGGTGATGCTGGCGATACCTCCCGCGTTCCTCGACGTACTTCGCCT
TCACGCTGGGGCAGCGGTCGCCCTGTCGGTCGACGAGGGCCAGCTGGTTGTCGAACCGCAGGTGCGCCCTCGCTACACGC
TGGATGAACTGCTGGCTCAGTGTGACGCCTCAGCCGACCCCACGGTTGAAGATCGGGAATGGCTCGATGGCAAACCGGTC
GGCGGTGAGTTGCTCTGA