Detailed information of TA system TA036352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 471609..472259
• Replicon: chromosome
• Accession: NZ_AP022612
• Organism: Mycolicibacterium confluentis strain JCM 13671

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N34_RS02145
• Protein ID: WP_085155320.1
• Coordinates: 471822..472259 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: G6N34_RS02140
• Protein ID: WP_085155322.1
• Coordinates: 471609..471809 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N34_RS02130	467595..469163	+	1569	WP_085155325.1	serine hydrolase	-
G6N34_RS02135	469160..471556	+	2397	WP_085155323.1	cation-translocating P-type ATPase	-
G6N34_RS02140	471609..471809	+	201	WP_085155322.1	antitoxin	Antitoxin
G6N34_RS02145	471822..472259	+	438	WP_085155320.1	SRPBCC family protein	Toxin
G6N34_RS02150	472365..473891	-	1527	WP_085155459.1	GAF domain-containing protein	-
G6N34_RS02155	473932..474582	-	651	WP_085155318.1	response regulator transcription factor	-
G6N34_RS02160	474747..475583	+	837	WP_163645293.1	hypothetical protein	-
G6N34_RS02165	475607..475990	+	384	WP_085155314.1	phage holin family protein	-
G6N34_RS02170	475987..476424	-	438	WP_085155312.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-143)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15818.40 Da
• Isoelectric Point: 10.0650

>T36352 WP_085155320.1 NZ_AP022612:471822-472259 [Mycolicibacterium confluentis]
MAKLSLSVDVPLPPEKAWEYASDLSRFKEWLTIHRVWRSPLPETLEKGTTLDSIVEVKGMANRVKWTIVNYKPPQSMTLN
GDGRGGVKIKLIAKIKPADDGSSVVTFDTHLGGPALFGPIGMVVAGALKGDIRESLNNFVKVFAS

Nucleotide

• Length: 438 bp

>T36352 NZ_AP022612:471822-472259 [Mycolicibacterium confluentis]
ATGGCGAAGCTGTCTCTGTCCGTGGACGTGCCGCTCCCACCCGAGAAGGCGTGGGAGTACGCGTCGGATCTCTCCCGCTT
CAAGGAGTGGCTGACCATCCACCGGGTCTGGCGCAGCCCACTGCCCGAGACCCTGGAGAAGGGCACGACGCTCGACTCGA
TCGTCGAGGTCAAGGGCATGGCCAACCGGGTCAAGTGGACGATCGTGAACTACAAACCGCCGCAGTCGATGACCCTCAAC
GGGGACGGCCGCGGTGGCGTGAAGATCAAGCTGATCGCCAAGATCAAGCCCGCGGACGACGGGTCGTCGGTGGTCACCTT
CGACACGCACCTGGGCGGTCCGGCGCTGTTCGGCCCGATCGGCATGGTGGTCGCGGGCGCGCTCAAGGGCGACATCCGCG
AGTCGCTGAACAACTTCGTGAAGGTCTTCGCGAGCTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7012.82 Da
• Isoelectric Point: 5.8041

>AT36352 WP_085155322.1 NZ_AP022612:471609-471809 [Mycolicibacterium confluentis]
MSFLDKAKDLIGKNTEKVEQAIDKAGDFVDDKTGGKHSATVDKVQDAAKNAAEKLKDTPGNDKPPA

Nucleotide

• Length: 201 bp

>AT36352 NZ_AP022612:471609-471809 [Mycolicibacterium confluentis]
ATGTCATTCCTGGACAAGGCAAAGGACCTGATCGGCAAGAACACCGAGAAGGTTGAGCAGGCCATCGACAAGGCCGGCGA
CTTCGTCGACGACAAGACCGGGGGCAAACACTCCGCCACCGTCGACAAGGTGCAGGACGCGGCCAAGAACGCCGCAGAGA
AGCTCAAGGACACCCCCGGCAACGACAAGCCGCCCGCCTGA