Detailed information of TA system TA036351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3465255..3465877
• Replicon: chromosome
• Accession: NZ_AP022610
• Organism: Mycolicibacterium madagascariense strain JCM 13574

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N60_RS16270
• Protein ID: WP_163739218.1
• Coordinates: 3465473..3465877 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N60_RS16265
• Protein ID: WP_163739216.1
• Coordinates: 3465255..3465476 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N60_RS16250	3462254..3463621	+	1368	WP_163744057.1	esterase family protein	-
G6N60_RS16255	3463762..3464001	+	240	WP_163744059.1	hypothetical protein	-
G6N60_RS16260	3464004..3465167	+	1164	WP_163739214.1	winged helix DNA-binding domain-containing protein	-
G6N60_RS16265	3465255..3465476	+	222	WP_163739216.1	antitoxin	Antitoxin
G6N60_RS16270	3465473..3465877	+	405	WP_163739218.1	PIN domain nuclease	Toxin
G6N60_RS16275	3465933..3468824	-	2892	WP_163739220.1	excinuclease ABC subunit UvrA	-
G6N60_RS16280	3468928..3469605	+	678	WP_163739222.1	MBL fold metallo-hydrolase	-
G6N60_RS16285	3469689..3470192	+	504	WP_163739223.1	DUF427 domain-containing protein	-
G6N60_RS16290	3470208..3470651	-	444	WP_163739225.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14898.14 Da
• Isoelectric Point: 5.2145

>T36351 WP_163739218.1 NZ_AP022610:3465473-3465877 [Mycolicibacterium madagascariense]
MSETLWLIDKSALVRLAESADVERWNNRVERGLVHISTVTRLEIGYSARSGPLARRNFREPPLSAMPVQYLTPAIEDRAL
EVQLLLADVGKHRAPSIPDLLIAATAELAGLTVLHLDKDFDVISDLTGQMTERL

Nucleotide

• Length: 405 bp

>T36351 NZ_AP022610:3465473-3465877 [Mycolicibacterium madagascariense]
ATGAGCGAGACGCTGTGGCTCATCGACAAGTCTGCACTGGTGCGGTTGGCCGAGAGCGCCGACGTCGAGCGTTGGAACAA
TCGCGTCGAGCGCGGCCTCGTCCACATCAGCACGGTGACCCGGCTCGAGATCGGCTACTCGGCACGCAGCGGCCCCCTCG
CCAGACGCAACTTTCGCGAACCACCGCTTTCCGCCATGCCGGTTCAATATCTCACTCCCGCAATCGAAGACCGCGCGCTC
GAGGTTCAACTTCTGCTCGCTGACGTCGGCAAGCACCGCGCGCCCTCGATTCCAGACTTGCTGATCGCGGCCACCGCCGA
ATTGGCCGGTCTGACCGTGCTACACCTGGACAAGGACTTCGACGTCATCAGCGATCTCACGGGTCAAATGACAGAACGCC
TTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8187.22 Da
• Isoelectric Point: 5.0028

>AT36351 WP_163739216.1 NZ_AP022610:3465255-3465476 [Mycolicibacterium madagascariense]
MSDLLIRDVPDDVLAALDKHAVRLGLSRTEYVRRRLAQDAHTATVNVTTADWRRIADDLADLGDAEVMGQAWR

Nucleotide

• Length: 222 bp

>AT36351 NZ_AP022610:3465255-3465476 [Mycolicibacterium madagascariense]
ATGAGCGACCTCCTCATAAGGGATGTTCCCGACGATGTCTTGGCGGCTCTCGACAAGCACGCCGTCAGGCTCGGGCTATC
CCGAACCGAATACGTCAGACGCCGACTGGCACAGGACGCGCACACGGCAACGGTGAACGTCACGACCGCCGACTGGCGGC
GAATCGCCGATGACTTGGCCGATCTCGGCGACGCCGAGGTGATGGGCCAAGCCTGGCGATGA