Detailed information of TA system TA036350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 865535..866015
• Replicon: chromosome
• Accession: NZ_AP022610
• Organism: Mycolicibacterium madagascariense strain JCM 13574

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: G6N60_RS03990
• Protein ID: WP_163732864.1
• Coordinates: 865535..865798 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: G6N60_RS03995
• Protein ID: WP_163732867.1
• Coordinates: 865788..866015 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N60_RS03975	861953..862765	+	813	WP_163732855.1	SDR family oxidoreductase	-
G6N60_RS03980	862824..863690	+	867	WP_163732858.1	carbon-nitrogen hydrolase family protein	-
G6N60_RS03985	863662..865404	-	1743	WP_163732861.1	amidohydrolase family protein	-
G6N60_RS03990	865535..865798	+	264	WP_163732864.1	BrnT family toxin	Toxin
G6N60_RS03995	865788..866015	+	228	WP_163732867.1	CopG family transcriptional regulator	Antitoxin
G6N60_RS04000	866064..866579	+	516	WP_163732870.1	mycothiol transferase	-
G6N60_RS04005	866581..867162	+	582	WP_179969637.1	PA-phosphatase	-
G6N60_RS04010	867172..868683	+	1512	WP_163732873.1	aldehyde dehydrogenase family protein	-
G6N60_RS04015	868813..869676	+	864	WP_163732877.1	polyphosphate kinase 2	-
G6N60_RS04020	869707..870555	+	849	WP_163732879.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9930.19 Da
• Isoelectric Point: 6.2192

>T36350 WP_163732864.1 NZ_AP022610:865535-865798 [Mycolicibacterium madagascariense]
MLEFEFDPAKSVVNSAKHGIDFEAAQAIWRDTHRIEIPARTTDEPRWMVIGQAAGQCWSAVVTYRDGHVRIISVRRSRES
EIAIYEG

Nucleotide

• Length: 264 bp

>T36350 NZ_AP022610:865535-865798 [Mycolicibacterium madagascariense]
ATGCTCGAGTTCGAGTTCGATCCCGCGAAGAGCGTGGTCAATTCGGCCAAGCATGGTATCGATTTCGAAGCGGCGCAAGC
CATTTGGCGTGATACGCACCGCATCGAGATCCCAGCGCGGACGACGGACGAGCCGAGGTGGATGGTGATCGGTCAGGCAG
CCGGCCAATGCTGGTCGGCAGTGGTTACCTACCGAGACGGACACGTCCGGATCATCTCGGTACGCCGCTCCCGGGAAAGT
GAGATTGCGATCTATGAAGGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8600.66 Da
• Isoelectric Point: 5.6278

>AT36350 WP_163732867.1 NZ_AP022610:865788-866015 [Mycolicibacterium madagascariense]
MKAEEFDARFDAGEDVTHALDAARARRPGEEHRRVNVDFPAWMIVELDREATRLGVTRQSLIKVWIAERLDHSAA

Nucleotide

• Length: 228 bp

>AT36350 NZ_AP022610:865788-866015 [Mycolicibacterium madagascariense]
ATGAAGGCTGAGGAGTTCGACGCCCGGTTCGACGCCGGCGAGGACGTGACCCACGCTCTGGATGCCGCGCGTGCACGGCG
ACCCGGCGAAGAGCACCGCCGCGTCAACGTCGACTTCCCGGCGTGGATGATCGTCGAACTCGACCGTGAGGCCACCCGGC
TCGGCGTCACGCGCCAGTCGCTCATCAAGGTGTGGATCGCCGAGAGGCTCGACCACTCCGCGGCCTGA