Detailed information of TA system TA036336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2393583..2394206
• Replicon: chromosome
• Accession: NZ_AP022606
• Organism: Mycobacterium branderi strain JCM 12687

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N47_RS12400
• Protein ID: WP_083131352.1
• Coordinates: 2393766..2394206 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A1X1RPX4
• Locus tag: G6N47_RS12395
• Protein ID: WP_062540463.1
• Coordinates: 2393583..2393762 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N47_RS12370	2389148..2389570	+	423	WP_083131348.1	Hsp20 family protein	-
G6N47_RS12375	2389831..2390151	+	321	WP_083131349.1	J domain-containing protein	-
G6N47_RS12380	2390179..2391651	-	1473	WP_083131350.1	acetyl-CoA carboxyl transferase	-
G6N47_RS12385	2391693..2392424	+	732	WP_083131351.1	enoyl-CoA hydratase	-
G6N47_RS12390	2392429..2393556	+	1128	WP_139799443.1	MBL fold metallo-hydrolase	-
G6N47_RS12395	2393583..2393762	+	180	WP_062540463.1	antitoxin	Antitoxin
G6N47_RS12400	2393766..2394206	+	441	WP_083131352.1	SRPBCC family protein	Toxin
G6N47_RS12405	2394279..2395052	+	774	WP_083131462.1	VOC family protein	-
G6N47_RS12410	2395049..2395492	-	444	WP_083131353.1	cupin domain-containing protein	-
G6N47_RS12415	2395527..2395943	+	417	WP_083131354.1	hypothetical protein	-
G6N47_RS12420	2395929..2397446	-	1518	WP_083131355.1	carotenoid oxygenase family protein	-
G6N47_RS29305	2397656..2397859	-	204	WP_179966444.1	PIN domain-containing protein	-
G6N47_RS29310	2397845..2398030	-	186	Protein_2466	type II toxin-antitoxin system prevent-host-death family antitoxin	-
G6N47_RS12435	2398341..2398895	-	555	WP_083131463.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16034.84 Da
• Isoelectric Point: 10.0407

>T36336 WP_083131352.1 NZ_AP022606:2393766-2394206 [Mycobacterium branderi]
MAKVSGSIDVPLPTEQAWEHAADLSRYKEWLTIHRVWRSKLPEKLEKGTVVESIVEVKGMPNRIKWTIVHYKPPEAMTLN
GVGVGGVKVKLLAKIKPKGDGCEVNFDVHLGGPALFGPIGMIVAGALRGDIRESLDNFVKVFAPVR

Nucleotide

• Length: 441 bp

>T36336 NZ_AP022606:2393766-2394206 [Mycobacterium branderi]
ATGGCGAAAGTCTCCGGATCCATCGACGTACCCCTGCCGACCGAGCAGGCCTGGGAGCATGCCGCCGACCTCTCCCGCTA
CAAGGAGTGGCTGACCATCCACCGGGTATGGCGCAGCAAGCTACCCGAGAAGTTGGAGAAGGGCACCGTCGTCGAGTCGA
TCGTCGAAGTCAAGGGCATGCCGAACCGGATCAAGTGGACGATCGTGCATTACAAGCCGCCGGAAGCCATGACACTCAAC
GGAGTTGGCGTCGGCGGCGTCAAGGTCAAGCTGCTCGCCAAAATCAAGCCTAAGGGCGACGGCTGCGAGGTCAACTTCGA
CGTGCACCTCGGTGGCCCGGCGCTGTTCGGCCCGATCGGGATGATCGTTGCGGGGGCGCTGCGGGGCGACATCCGCGAGT
CGCTCGACAATTTCGTCAAGGTGTTCGCCCCGGTGCGATGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6293.02 Da
• Isoelectric Point: 4.9974

>AT36336 WP_062540463.1 NZ_AP022606:2393583-2393762 [Mycobacterium branderi]
MGFLDKAKSLLSQNADKVEQAIDKAGDIVDDKTGGKYKDAVDKVQDAAKKAVDKGNPEN

Nucleotide

• Length: 180 bp

>AT36336 NZ_AP022606:2393583-2393762 [Mycobacterium branderi]
ATGGGATTTTTGGACAAGGCCAAAAGCCTGCTGTCGCAGAACGCCGACAAGGTTGAACAGGCGATCGACAAGGCGGGCGA
CATCGTCGACGACAAAACCGGCGGCAAGTACAAGGACGCCGTGGACAAGGTGCAGGACGCGGCCAAAAAGGCTGTGGACA
AAGGCAACCCAGAGAACTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X1RPX4