Detailed information of TA system TA036335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 2228248..2228915
• Replicon: chromosome
• Accession: NZ_AP022606
• Organism: Mycobacterium branderi strain JCM 12687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N47_RS11525
• Protein ID: WP_083133948.1
• Coordinates: 2228523..2228915 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N47_RS11520
• Protein ID: WP_083133949.1
• Coordinates: 2228248..2228523 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N47_RS11485	2223348..2224214	+	867	WP_083133956.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-
G6N47_RS11490	2224198..2224461	-	264	WP_083133955.1	cytochrome C oxidase subunit IV family protein	-
G6N47_RS11495	2224458..2225042	-	585	WP_083133954.1	cytochrome c oxidase subunit 3 family protein	-
G6N47_RS11500	2225042..2225761	-	720	WP_083133953.1	hypothetical protein	-
G6N47_RS11505	2225758..2226495	-	738	WP_083133952.1	hypothetical protein	-
G6N47_RS11510	2226557..2227132	+	576	WP_083133951.1	TetR/AcrR family transcriptional regulator	-
G6N47_RS11515	2227133..2228194	+	1062	WP_083133950.1	phosphotransferase family protein	-
G6N47_RS11520	2228248..2228523	+	276	WP_083133949.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6N47_RS11525	2228523..2228915	+	393	WP_083133948.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N47_RS11530	2228912..2230513	-	1602	WP_083133947.1	FAD-dependent oxidoreductase	-
G6N47_RS11535	2230525..2231193	-	669	WP_083133946.1	helix-turn-helix domain-containing protein	-
G6N47_RS11540	2231293..2232162	+	870	WP_083133945.1	alpha/beta fold hydrolase	-
G6N47_RS11545	2232159..2233823	+	1665	WP_083133944.1	bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14442.68 Da
• Isoelectric Point: 9.6502

>T36335 WP_083133948.1 NZ_AP022606:2228523-2228915 [Mycobacterium branderi]
MAATYLDSSAVVKLAVREPESDALRRYLRSHRPWVSSALTRTEVMRAVLDKGEPARKAGRRALADLDLIRVDNRVLDLAG
ALLPSELRSLDAIHMATAQRLGNDLREVVTYDVRMTEAAELLGFRVMAPT

Nucleotide

• Length: 393 bp

>T36335 NZ_AP022606:2228523-2228915 [Mycobacterium branderi]
ATGGCAGCCACCTACCTCGATTCGTCCGCCGTCGTCAAGCTCGCAGTGCGTGAGCCGGAGTCGGACGCCCTGCGTCGCTA
CCTTCGGAGCCACCGGCCGTGGGTATCGAGCGCTCTTACTCGGACGGAAGTCATGCGGGCGGTCCTCGACAAGGGCGAAC
CGGCGCGGAAAGCCGGACGCCGAGCGCTCGCTGATTTAGACCTAATTCGCGTCGATAATCGAGTGCTTGATCTTGCTGGC
GCACTCCTGCCATCCGAACTGCGCTCGCTCGATGCGATTCACATGGCTACGGCGCAGCGGCTCGGCAACGACCTCCGCGA
GGTTGTTACTTACGATGTGCGTATGACGGAGGCCGCCGAGTTGCTGGGCTTTCGCGTTATGGCACCGACCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10065.43 Da
• Isoelectric Point: 4.3854

>AT36335 WP_083133949.1 NZ_AP022606:2228248-2228523 [Mycobacterium branderi]
MSSVGVRELRQRASELLRRVEAGETIEITDRGRPVALLSPMPEGSPYERMLASGEIEPATGSLDDLPEPIELEPGVELPS
VTLARLRERER

Nucleotide

• Length: 276 bp

>AT36335 NZ_AP022606:2228248-2228523 [Mycobacterium branderi]
ATGAGTTCGGTGGGTGTGCGTGAGCTTCGGCAGCGGGCAAGCGAACTCCTCCGGCGCGTCGAGGCCGGTGAAACGATTGA
GATCACCGATCGCGGTCGGCCCGTCGCCCTGCTCTCGCCGATGCCGGAGGGCAGCCCATACGAGCGGATGTTGGCAAGCG
GCGAGATCGAGCCCGCGACCGGAAGCCTTGACGATTTGCCCGAGCCGATCGAGCTAGAACCGGGTGTCGAGCTGCCATCG
GTAACGCTCGCGCGCCTGCGTGAGCGCGAGCGTTGA