Detailed information of TA system TA036289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2756860..2757513
• Replicon: chromosome
• Accession: NZ_AP022597
• Organism: Mycobacterium paraintracellulare strain JCM 30622

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N00_RS13830
• Protein ID: WP_008253911.1
• Coordinates: 2757052..2757513 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: I2A997
• Locus tag: G6N00_RS13825
• Protein ID: WP_008253910.1
• Coordinates: 2756860..2757048 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N00_RS13815	2752833..2754410	+	1578	WP_014383898.1	serine hydrolase	-
G6N00_RS13820	2754407..2756794	+	2388	WP_014383899.1	cation-translocating P-type ATPase	-
G6N00_RS13825	2756860..2757048	+	189	WP_008253910.1	antitoxin	Antitoxin
G6N00_RS13830	2757052..2757513	+	462	WP_008253911.1	SRPBCC family protein	Toxin
G6N00_RS13835	2757598..2758368	+	771	WP_008253913.1	VOC family protein	-
G6N00_RS13840	2758442..2758876	+	435	WP_008253914.1	hypothetical protein	-
G6N00_RS13845	2758881..2759369	-	489	WP_008253915.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
G6N00_RS13850	2759369..2760895	-	1527	WP_014381862.1	carotenoid oxygenase family protein	-
G6N00_RS13855	2760897..2762117	-	1221	WP_009957327.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T36289 WP_008253911.1 NZ_AP022597:2757052-2757513 [Mycobacterium paraintracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T36289 NZ_AP022597:2757052-2757513 [Mycobacterium paraintracellulare]
ATGGCCAAGCTTTCCGGATCCATCGATGTCCCGCTGCCGCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGATTGGCTGACCATCCACCGGGTGTGGCGCAGCGCATTGCCCGACGAGATCGAGAAGGGCTCGGTCGTCGAGTCGA
TTGTCGAGGTCAAGGGCATGCCCAACCGGATCAAATGGACGGTCGTGCGCTACAAGCCACCCGAGGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGATCCAGCCCAAAGGGGACGGCTCCCTCGTCAGTTTCGA
CGTGCACCTCGGTGGCCCCGCACTGTTCGGCCCCATCGGCATGATCGTCGCCGCCGCACTGCGCGGCGATATCGACCAGT
CGCTGGAGAACTTCGTGACGGTGTTCACCCGGCCCGATCCGAGCAGCAACGGGCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6797.44 Da
• Isoelectric Point: 4.4702

>AT36289 WP_008253910.1 NZ_AP022597:2756860-2757048 [Mycobacterium paraintracellulare]
MGFLDKAKDLLWQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT36289 NZ_AP022597:2756860-2757048 [Mycobacterium paraintracellulare]
ATGGGATTCCTGGACAAGGCCAAGGATCTGTTGTGGCAGAACGCCGACAAGGTCGAGACGGCCATCGACAAGGCAGGCGA
GTTCGTCGACGAAAAGACGCAGGGCAAATACTCCGACACCATTCACCAGGTGCAGGAGCAGGCGAAGAAGGCGGCCGGCG
CCGCGGACACGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7TPT2