Detailed information of TA system TA036284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3340451..3341277
• Replicon: chromosome
• Accession: NZ_AP022595
• Organism: Mycolicibacterium sarraceniae strain JCM 30395

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: G6N13_RS16675
• Protein ID: WP_163698746.1
• Coordinates: 3340723..3341277 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: G6N13_RS16670
• Protein ID: WP_163698744.1
• Coordinates: 3340451..3340726 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N13_RS16645	3335778..3337616	+	1839	WP_163698738.1	long-chain fatty acid--CoA ligase	-
G6N13_RS16650	3337619..3338110	+	492	WP_163698740.1	hypothetical protein	-
G6N13_RS24345	3338422..3338643	-	222	WP_179964998.1	hypothetical protein	-
G6N13_RS24350	3338747..3338887	-	141	WP_179964999.1	hypothetical protein	-
G6N13_RS16660	3339014..3339523	+	510	WP_163698742.1	hypothetical protein	-
G6N13_RS16665	3340011..3340259	+	249	Protein_3286	IS1634 family transposase	-
G6N13_RS16670	3340451..3340726	+	276	WP_163698744.1	DUF1778 domain-containing protein	Antitoxin
G6N13_RS16675	3340723..3341277	+	555	WP_163698746.1	GNAT family N-acetyltransferase	Toxin
G6N13_RS16700	3341980..3342255	-	276	WP_163702217.1	hypothetical protein	-
G6N13_RS16705	3342330..3342746	-	417	WP_163702219.1	hypothetical protein	-
G6N13_RS16710	3342866..3343679	+	814	Protein_3291	IS1595 family transposase	-
G6N13_RS16715	3343781..3345139	-	1359	WP_163698748.1	cytochrome P450	-
G6N13_RS16720	3345451..3345840	+	390	WP_163698750.1	STAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3343027..3343653	626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-82)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20199.86 Da
• Isoelectric Point: 7.3728

>T36284 WP_163698746.1 NZ_AP022595:3340723-3341277 [Mycolicibacterium sarraceniae]
VSRYSLPRPITDSDDSTTFDSGASSLDSYLRKRALANHVTGASRCFVTCCDGRVVGYYALATGAVSHDGCSGRFRRNMPD
PIPVILLSRLAIDVKHQHRGLGESLLRDAIARSVLVAEQVGVRAILVHALHDEARQFYLRYGFEPSPTDPLQLMLLVKDV
KASVASLRGENDAADQRGEFIDRD

Nucleotide

• Length: 555 bp

>T36284 NZ_AP022595:3340723-3341277 [Mycolicibacterium sarraceniae]
GTGAGCCGCTACAGCCTCCCGCGGCCGATCACTGACAGCGACGACTCCACCACATTCGACAGCGGCGCGTCCAGCCTGGA
CTCGTATCTGCGCAAGAGGGCTCTGGCCAACCATGTCACCGGTGCATCTCGTTGCTTCGTCACATGCTGCGATGGTCGCG
TCGTCGGCTACTACGCGCTCGCAACGGGAGCGGTGTCGCACGATGGCTGCAGCGGAAGATTTCGTCGCAACATGCCTGAT
CCGATCCCGGTGATCCTGTTGTCGCGCCTGGCTATCGACGTCAAGCACCAGCACCGTGGGCTGGGTGAGAGCCTCCTCCG
CGATGCCATTGCGCGCAGCGTCTTAGTCGCCGAGCAAGTAGGTGTTCGCGCCATCCTCGTGCATGCACTTCACGACGAGG
CCCGTCAGTTCTACCTCCGCTACGGGTTCGAGCCGTCGCCCACCGACCCACTGCAACTGATGCTGCTGGTCAAAGACGTC
AAGGCGAGCGTGGCGAGCTTGCGGGGAGAAAACGACGCGGCCGACCAACGCGGCGAATTTATCGACAGGGATTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10192.63 Da
• Isoelectric Point: 6.2191

>AT36284 WP_163698744.1 NZ_AP022595:3340451-3340726 [Mycolicibacterium sarraceniae]
MATKSERFAVRLTPAQDALIRHAAEVEGIDLTTFTVSATVAHARDVLADRRHFQLDDAAWTEFNALLERPVQYKPALAKL
FAKPSLFDPEL

Nucleotide

• Length: 276 bp

>AT36284 NZ_AP022595:3340451-3340726 [Mycolicibacterium sarraceniae]
ATGGCTACGAAGTCCGAACGATTCGCGGTGCGCCTGACTCCCGCGCAGGACGCACTGATTCGCCATGCCGCCGAGGTCGA
AGGCATCGATCTCACCACGTTCACCGTGAGTGCGACCGTCGCTCACGCCCGCGACGTGCTCGCAGATCGCCGGCACTTTC
AGCTCGACGACGCGGCATGGACCGAGTTCAACGCGTTGCTCGAGCGCCCCGTTCAGTACAAGCCTGCGTTGGCGAAGCTG
TTCGCGAAGCCATCGCTGTTCGATCCCGAGCTGTGA