Detailed information of TA system TA036250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3481673..3482277
• Replicon: chromosome
• Accession: NZ_AP022583
• Organism: Mycobacterium noviomagense strain JCM 16367

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N15_RS16455
• Protein ID: WP_083087678.1
• Coordinates: 3481885..3482277 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N15_RS16450
• Protein ID: WP_083087741.1
• Coordinates: 3481673..3481888 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N15_RS16420	3476814..3477314	+	501	WP_083087675.1	MarR family winged helix-turn-helix transcriptional regulator	-
G6N15_RS16425	3477689..3477841	+	153	WP_083087739.1	AAA family ATPase	-
G6N15_RS16430	3477888..3479053	+	1166	Protein_3257	IS3 family transposase	-
G6N15_RS16435	3479705..3480196	+	492	WP_083087676.1	MarR family winged helix-turn-helix transcriptional regulator	-
G6N15_RS16440	3480543..3480806	+	264	WP_083087740.1	hypothetical protein	-
G6N15_RS16445	3480846..3481592	-	747	WP_083087677.1	enoyl-CoA hydratase	-
G6N15_RS16450	3481673..3481888	+	216	WP_083087741.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G6N15_RS16455	3481885..3482277	+	393	WP_083087678.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N15_RS16460	3482299..3482565	+	267	WP_083087679.1	DUF2277 family protein	-
G6N15_RS16465	3482562..3483122	+	561	WP_083087680.1	DUF1802 family protein	-
G6N15_RS16470	3483132..3484748	-	1617	WP_083087681.1	CocE/NonD family hydrolase	-
G6N15_RS16475	3484840..3485037	-	198	Protein_3266	DUF3558 domain-containing protein	-
G6N15_RS16480	3485223..3486212	+	990	WP_083087682.1	metallophosphoesterase	-
G6N15_RS16485	3486209..3486886	+	678	WP_083087683.1	4'-phosphopantetheinyl transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	esxN / esxN	3445305..3502228	56923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-51)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14612.70 Da
• Isoelectric Point: 7.5750

>T36250 WP_083087678.1 NZ_AP022583:3481885-3482277 [Mycobacterium noviomagense]
MTTILLDSHVVHWWSAEPRRLSQAASGAIEQANELAVAAITWFELAWLAEHERIQLTIPVTSWLQQLADHVRTVGITPAV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRQVTVW

Nucleotide

• Length: 393 bp

>T36250 NZ_AP022583:3481885-3482277 [Mycobacterium noviomagense]
ATGACCACGATTCTGCTCGATTCCCATGTGGTGCATTGGTGGTCGGCCGAGCCCCGGCGTCTGAGTCAGGCTGCAAGCGG
GGCCATCGAACAGGCCAATGAGCTTGCGGTTGCCGCCATTACGTGGTTCGAACTGGCGTGGCTTGCCGAACACGAACGCA
TCCAACTGACGATTCCGGTGACATCCTGGCTTCAGCAGCTCGCCGATCACGTCCGCACCGTGGGCATCACTCCAGCCGTC
GCTGCCACCGCGGTGGCGCTGCCCTCGTCGTTTCCTGGCGATCCTGCAGACCGGTTAATTTATGCCACGGCTATTGAACA
TGGCTGGCGTCTAGTAACCAAGGACCGGCGGCTGCGGAGCCACCGCCATCCGCGCCAGGTCACGGTGTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7447.55 Da
• Isoelectric Point: 5.6257

>AT36250 WP_083087741.1 NZ_AP022583:3481673-3481888 [Mycobacterium noviomagense]
MTATEVKAKILALLDEVAGGEEIEITKHGRTVARLIGATGSHALKGRLSNVAMTAVDEEALFSTGVSWNLP

Nucleotide

• Length: 216 bp

>AT36250 NZ_AP022583:3481673-3481888 [Mycobacterium noviomagense]
ATGACCGCTACCGAAGTGAAGGCGAAGATCCTTGCCTTGCTCGACGAGGTGGCGGGAGGCGAAGAAATCGAAATCACCAA
GCACGGCCGTACGGTGGCTCGGCTGATAGGAGCGACCGGTTCTCACGCGCTCAAGGGCCGGTTATCCAATGTGGCGATGA
CGGCCGTCGACGAAGAGGCGCTCTTCAGTACCGGGGTGTCCTGGAACCTGCCATGA