Detailed information of TA system TA036232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2102395..2103050
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N24_RS09665
• Protein ID: WP_179963478.1
• Coordinates: 2102652..2103050 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N24_RS09660
• Protein ID: WP_085162009.1
• Coordinates: 2102395..2102655 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS09630	2097621..2098169	-	549	WP_085162024.1	thioredoxin family protein	-
G6N24_RS09635	2098295..2099152	+	858	WP_085162013.1	PPOX class F420-dependent oxidoreductase	-
G6N24_RS09640	2099222..2100928	+	1707	WP_085162012.1	PecA family PE domain-processing aspartic protease	-
G6N24_RS09645	2100939..2101601	-	663	WP_139822524.1	cutinase family protein	-
G6N24_RS09650	2101686..2101922	+	237	WP_085162023.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
G6N24_RS09655	2101919..2102341	+	423	WP_085162010.1	type II toxin-antitoxin system VapC family toxin	-
G6N24_RS09660	2102395..2102655	+	261	WP_085162009.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6N24_RS09665	2102652..2103050	+	399	WP_179963478.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N24_RS09670	2103088..2104272	-	1185	WP_163745473.1	cyclic nucleotide-binding domain-containing protein	-
G6N24_RS09675	2104247..2105407	-	1161	WP_163745474.1	hypothetical protein	-
G6N24_RS09680	2105840..2106085	+	246	WP_085162006.1	Arc family DNA-binding protein	-
G6N24_RS09685	2106082..2106510	+	429	WP_085162005.1	type II toxin-antitoxin system VapC family toxin	-
G6N24_RS09690	2106574..2107752	-	1179	WP_085162004.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 13942.85 Da
• Isoelectric Point: 4.2977

>T36232 WP_179963478.1 NZ_AP022581:2102652-2103050 [Mycobacterium lacus]
VTSAGVLDTSVFIAQESGRLPDASRLPDELATTVVTVAELNLGVLAAASADIRAQRLATLDATADIVAFPVDDDAARMWA
RLRIHLAEAGRRVRINDLWIAAIAASRGLPVVTQDDDFDPLDGVAGLTIVRV

Nucleotide

• Length: 399 bp

>T36232 NZ_AP022581:2102652-2103050 [Mycobacterium lacus]
GTGACCTCCGCCGGCGTGCTCGATACGTCAGTATTCATCGCGCAGGAGAGCGGTCGCCTGCCCGATGCGTCGCGGCTCCC
CGACGAACTGGCGACCACCGTCGTCACCGTGGCCGAGCTCAACCTCGGCGTGCTCGCCGCCGCCTCGGCGGACATTCGAG
CGCAACGACTGGCAACACTGGACGCTACTGCCGACATCGTCGCATTTCCGGTCGACGACGACGCAGCGCGGATGTGGGCG
CGGCTTCGAATCCATCTCGCAGAAGCCGGTCGGCGAGTCCGCATCAACGACCTTTGGATCGCCGCCATCGCAGCTTCCCG
GGGACTGCCGGTGGTGACGCAAGACGATGACTTCGATCCCCTCGACGGGGTCGCCGGCTTGACGATTGTGCGTGTCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9554.91 Da
• Isoelectric Point: 9.1203

>AT36232 WP_085162009.1 NZ_AP022581:2102395-2102655 [Mycobacterium lacus]
MSEVASRELRNHTAGLLRRVQAGEDITVTVNGKAVALLTALRPTRRRWLGRDELLRRLERAQADLALRQELAELAGDTSD
ELGPLP

Nucleotide

• Length: 261 bp

>AT36232 NZ_AP022581:2102395-2102655 [Mycobacterium lacus]
ATGTCGGAAGTTGCCTCCCGAGAACTGCGCAATCACACCGCAGGGCTGCTCCGGCGCGTGCAGGCTGGTGAAGATATCAC
CGTCACCGTCAACGGCAAGGCGGTCGCCCTTCTCACCGCTCTGCGGCCGACCCGACGCAGATGGTTAGGCCGTGACGAAC
TACTGCGGAGGCTCGAGCGCGCGCAAGCGGATCTTGCGTTGCGCCAGGAACTCGCTGAGCTAGCAGGGGACACCTCCGAC
GAGCTTGGTCCGCTGCCGTGA