Detailed information of TA system TA036230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1745653..1746266
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N24_RS07995
• Protein ID: WP_085160315.1
• Coordinates: 1745653..1746042 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N24_RS08000
• Protein ID: WP_085160318.1
• Coordinates: 1746039..1746266 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS07975	1742588..1742830	+	243	WP_163745454.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
G6N24_RS07980	1742812..1743099	-	288	WP_139822387.1	DUF4189 domain-containing protein	-
G6N24_RS07985	1743337..1743915	-	579	WP_085160311.1	cyclic nucleotide-binding domain-containing protein	-
G6N24_RS07990	1744023..1745579	-	1557	WP_085160313.1	succinate-semialdehyde dehydrogenase (NADP(+))	-
G6N24_RS07995	1745653..1746042	-	390	WP_085160315.1	PIN domain-containing protein	Toxin
G6N24_RS08000	1746039..1746266	-	228	WP_085160318.1	antitoxin	Antitoxin
G6N24_RS08005	1746361..1747035	-	675	WP_139822389.1	glycoside hydrolase	-
G6N24_RS08010	1747019..1747324	+	306	WP_085160347.1	DUF167 domain-containing protein	-
G6N24_RS08015	1747335..1748411	-	1077	WP_085160320.1	PDR/VanB family oxidoreductase	-
G6N24_RS08020	1748422..1749357	-	936	WP_179963474.1	alpha/beta fold hydrolase	-
G6N24_RS08025	1749377..1750285	-	909	WP_085160322.1	metal-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13876.89 Da
• Isoelectric Point: 6.6037

>T36230 WP_085160315.1 NZ_AP022581:c1746042-1745653 [Mycobacterium lacus]
MIVLDASAAVELVLATGSGAAVARRLRGEALHAPAHFDVEAIGAIRRAVIRRLLSDHEGLVSVADFQSLPVRRWPTKALA
QRAYQLRHTHTVADGMYVALAEGLEAPLVTCDERLAQSHGHDADIELLA

Nucleotide

• Length: 390 bp

>T36230 NZ_AP022581:c1746042-1745653 [Mycobacterium lacus]
ATGATCGTGTTGGACGCCTCGGCTGCCGTCGAGCTGGTGCTCGCGACGGGCTCGGGCGCCGCGGTCGCCCGGCGCCTGCG
CGGTGAAGCACTGCATGCCCCTGCCCATTTCGATGTGGAGGCGATCGGCGCGATACGCCGAGCGGTCATCCGCCGGCTGC
TCAGCGACCATGAAGGACTCGTCAGCGTTGCCGACTTCCAAAGCCTGCCGGTTCGGCGTTGGCCCACAAAAGCACTTGCC
CAGCGCGCCTACCAATTACGACACACTCATACCGTGGCCGACGGAATGTACGTCGCCCTCGCCGAGGGCCTCGAGGCGCC
GCTCGTCACGTGCGACGAGCGACTCGCTCAATCCCATGGCCATGACGCAGACATCGAGCTGCTTGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8255.49 Da
• Isoelectric Point: 4.5535

>AT36230 WP_085160318.1 NZ_AP022581:c1746266-1746039 [Mycobacterium lacus]
MPMMVQIRNVPDELVHELKARAAAQRMSLSDFLLARLGEIAGEPTLDDVLDRLASLPRRDLGTNAAELVAEARSE

Nucleotide

• Length: 228 bp

>AT36230 NZ_AP022581:c1746266-1746039 [Mycobacterium lacus]
ATGCCAATGATGGTTCAGATCCGCAATGTGCCCGATGAACTTGTCCACGAACTGAAGGCCCGAGCGGCGGCACAGCGGAT
GAGCCTCAGCGATTTCCTGCTTGCCCGGCTCGGCGAAATCGCCGGAGAGCCGACCCTCGACGACGTCCTCGACCGCCTCG
CGTCGCTGCCGCGCCGCGACCTCGGCACGAACGCCGCCGAATTGGTCGCCGAAGCCCGATCCGAATGA