Detailed information of TA system TA036225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1202287..1202903
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N24_RS05665
• Protein ID: WP_085156729.1
• Coordinates: 1202586..1202903 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N24_RS05660
• Protein ID: WP_139822198.1
• Coordinates: 1202287..1202586 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS05655	1200751..1202091	-	1341	WP_085156735.1	4-aminobutyrate--2-oxoglutarate transaminase	-
G6N24_RS05660	1202287..1202586	+	300	WP_139822198.1	antitoxin	Antitoxin
G6N24_RS05665	1202586..1202903	+	318	WP_085156729.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N24_RS05670	1203093..1203446	+	354	WP_085156726.1	preprotein translocase subunit YajC	-
G6N24_RS05675	1203528..1205285	+	1758	WP_163745421.1	protein translocase subunit SecD	-
G6N24_RS05680	1205290..1206560	+	1271	Protein_1118	protein translocase subunit SecF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11271.91 Da
• Isoelectric Point: 6.2052

>T36225 WP_085156729.1 NZ_AP022581:1202586-1202903 [Mycobacterium lacus]
MRPIHVAQLDKARPVLILTREVVRPHLANVTVAPITTTSRGLATEVPVGAVNGLDQPSVVSCDNIQTIPVSDLGRHIGYL
FASQEPALAEAIGNAFDLDWQPSPR

Nucleotide

• Length: 318 bp

>T36225 NZ_AP022581:1202586-1202903 [Mycobacterium lacus]
ATGCGACCTATCCACGTCGCCCAGCTTGACAAGGCCAGACCCGTCCTGATTCTCACCCGCGAGGTCGTGCGTCCACACCT
GGCCAACGTCACTGTCGCGCCAATTACGACGACCAGCCGTGGGCTGGCAACCGAGGTCCCGGTAGGCGCGGTGAACGGGC
TCGATCAACCATCGGTGGTCAGTTGCGACAACATCCAAACGATCCCGGTGTCTGACCTTGGTCGTCACATTGGCTACCTC
TTCGCGTCGCAGGAGCCGGCCCTGGCTGAAGCCATCGGCAACGCGTTCGACCTCGACTGGCAACCCTCACCGCGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10872.30 Da
• Isoelectric Point: 8.0548

>AT36225 WP_139822198.1 NZ_AP022581:1202287-1202586 [Mycobacterium lacus]
MRADGRCIRRSGAYQSGTIREMSTQIAVRLPDEIVAFIDDEVRGRHARSRAAVVLRALERERRRRLAERDAEILAAAMSA
PSDLDALAGHSAGTALDID

Nucleotide

• Length: 300 bp

>AT36225 NZ_AP022581:1202287-1202586 [Mycobacterium lacus]
CTGCGAGCCGACGGTAGGTGTATTCGGCGCTCGGGAGCATACCAAAGTGGTACCATCCGTGAAATGAGTACCCAGATTGC
GGTCCGCCTGCCCGACGAGATCGTCGCCTTCATCGACGACGAGGTCAGGGGGCGGCATGCCCGTAGCCGTGCGGCCGTCG
TGCTCAGGGCGCTGGAACGGGAACGTCGCCGTCGTCTCGCCGAACGGGACGCGGAGATTCTCGCGGCCGCTATGTCAGCG
CCGAGCGATCTCGATGCGCTGGCTGGCCACTCTGCTGGTACCGCCCTCGACATCGACTGA