Detailed information of TA system TA036224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1186069..1186609
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N24_RS05570
• Protein ID: WP_085156790.1
• Coordinates: 1186069..1186377 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N24_RS05575
• Protein ID: WP_139822201.1
• Coordinates: 1186364..1186609 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS05525	1181706..1182461	+	756	WP_085156813.1	YebC/PmpR family DNA-binding transcriptional regulator	-
G6N24_RS05530	1182523..1182768	+	246	WP_085156810.1	hypothetical protein	-
G6N24_RS05535	1182755..1183159	+	405	WP_085156807.1	type II toxin-antitoxin system VapC family toxin	-
G6N24_RS05540	1183425..1183829	+	405	WP_085156805.1	PE family protein	-
G6N24_RS05545	1184025..1184318	+	294	WP_085156802.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
G6N24_RS05550	1184315..1184716	+	402	WP_085156799.1	PIN domain-containing protein	-
G6N24_RS05555	1184834..1185274	-	441	WP_085156796.1	type II toxin-antitoxin system VapC family toxin	-
G6N24_RS05560	1185261..1185548	-	288	WP_085156793.1	antitoxin	-
G6N24_RS05565	1185818..1185925	+	108	Protein_1095	PE domain-containing protein	-
G6N24_RS05570	1186069..1186377	-	309	WP_085156790.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N24_RS05575	1186364..1186609	-	246	WP_139822201.1	ribbon-helix-helix protein, CopG family	Antitoxin
G6N24_RS05580	1187081..1187368	+	288	WP_085156781.1	PE family protein	-
G6N24_RS05585	1187526..1187762	-	237	WP_085156776.1	hypothetical protein	-
G6N24_RS05590	1187801..1189369	-	1569	WP_085156773.1	polyamine aminopropyltransferase	-
G6N24_RS05595	1189366..1189821	-	456	WP_085156770.1	DUF350 domain-containing protein	-
G6N24_RS05600	1189846..1190277	-	432	WP_085156767.1	DUF4247 domain-containing protein	-
G6N24_RS05605	1190274..1190774	-	501	WP_085156764.1	DUF2617 family protein	-
G6N24_RS05610	1190782..1191390	-	609	WP_085156761.1	DUF4178 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11273.04 Da
• Isoelectric Point: 11.8634

>T36224 WP_085156790.1 NZ_AP022581:c1186377-1186069 [Mycobacterium lacus]
MRRGELWFAATPGGDRPVLILTRDPVADRIGAVVVAALTRTRRGLVSELALTVSDDRVPSDCVVNFDNIHTLPRSAFRRR
ITRLSPARLHQVCHTLRASTGC

Nucleotide

• Length: 309 bp

>T36224 NZ_AP022581:c1186377-1186069 [Mycobacterium lacus]
ATGCGCCGCGGTGAACTCTGGTTCGCCGCAACCCCCGGCGGCGACCGGCCCGTGCTCATCCTCACCCGAGATCCGGTCGC
AGACCGGATCGGCGCCGTCGTCGTCGCAGCACTGACCCGCACCCGCCGAGGACTGGTCTCCGAGCTGGCACTAACCGTCT
CCGACGACCGCGTGCCCAGCGACTGCGTGGTCAACTTCGACAACATCCACACCCTGCCGCGTTCCGCGTTTCGACGCCGC
ATCACCCGCTTATCCCCGGCACGGCTCCACCAGGTCTGCCACACCCTGCGCGCCAGCACCGGCTGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9247.08 Da
• Isoelectric Point: 4.2386

>AT36224 WP_139822201.1 NZ_AP022581:c1186609-1186364 [Mycobacterium lacus]
MISFRADDADAAEAERWARRLHIDRSEFLRDALRRRLAELAADQEAHAYAEQPLTDAEQALAQIADWGPAEDWADWADAP
R

Nucleotide

• Length: 246 bp

>AT36224 NZ_AP022581:c1186609-1186364 [Mycobacterium lacus]
ATGATCAGCTTTCGCGCGGACGACGCGGACGCGGCCGAAGCCGAGCGGTGGGCGCGGCGCCTGCACATCGACCGATCAGA
GTTCCTTCGCGATGCGTTGCGCCGCCGCCTGGCCGAGCTCGCCGCCGACCAAGAGGCGCACGCCTACGCCGAGCAACCGT
TGACAGACGCCGAGCAAGCCCTGGCCCAGATCGCCGACTGGGGACCCGCCGAAGACTGGGCCGACTGGGCCGATGCGCCG
CGGTGA