Detailed information of TA system TA036222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1062546..1063328
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: G6N24_RS04990
• Protein ID: WP_085158597.1
• Coordinates: 1062828..1063328 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: G6N24_RS04985
• Protein ID: WP_085158599.1
• Coordinates: 1062546..1062824 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS04945	1057557..1058222	+	666	WP_085158611.1	rhomboid family intramembrane serine protease	-
G6N24_RS04950	1058219..1059034	+	816	WP_085158609.1	glutamate racemase	-
G6N24_RS04955	1059109..1059882	+	774	WP_085158607.1	MBL fold metallo-hydrolase	-
G6N24_RS04960	1059969..1060748	+	780	WP_085158605.1	ribonuclease PH	-
G6N24_RS04965	1060745..1061365	+	621	WP_085158709.1	non-canonical purine NTP pyrophosphatase	-
G6N24_RS04970	1061362..1061718	-	357	WP_085158603.1	DUF3817 domain-containing protein	-
G6N24_RS04975	1061715..1062092	-	378	WP_085158601.1	hypothetical protein	-
G6N24_RS04985	1062546..1062824	+	279	WP_085158599.1	DUF1778 domain-containing protein	Antitoxin
G6N24_RS04990	1062828..1063328	+	501	WP_085158597.1	GNAT family N-acetyltransferase	Toxin
G6N24_RS04995	1063676..1064848	-	1173	WP_085158596.1	acyl-CoA dehydrogenase family protein	-
G6N24_RS05000	1064944..1065702	+	759	WP_085158594.1	3-oxoacyl-ACP reductase FabG	-
G6N24_RS05005	1065787..1067003	+	1217	Protein_985	acetyl-CoA C-acetyltransferase	-
G6N24_RS05010	1067000..1068211	+	1212	WP_085158592.1	CoA transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18123.85 Da
• Isoelectric Point: 9.1782

>T36222 WP_085158597.1 NZ_AP022581:1062828-1063328 [Mycobacterium lacus]
VSGYGLPRPISEADEAATFDSGERSLDDYLRKRALATHVAGASRCFVTCRDRRVVGFYAVAWASVERGAGAGGVRRNMPD
PVPVILLTRLAVDRKERGKGLGKHLLRDAIARCVGVAEQVGVRAMLVHALHDEARSFYLHFEFEPSPTDPLHLMLSMKDA
RALIGA

Nucleotide

• Length: 501 bp

>T36222 NZ_AP022581:1062828-1063328 [Mycobacterium lacus]
GTGAGCGGTTACGGCCTGCCGCGGCCGATCAGCGAGGCTGACGAGGCCGCCACGTTCGACAGCGGTGAACGCAGCTTGGA
TGACTATCTGCGCAAGCGGGCGTTGGCCACTCACGTTGCGGGGGCGTCGCGCTGTTTCGTGACGTGCCGGGATCGCCGGG
TGGTCGGTTTCTATGCGGTGGCGTGGGCATCGGTGGAGCGTGGTGCCGGTGCGGGGGGCGTGCGCCGCAACATGCCTGAC
CCGGTGCCGGTAATTTTGTTGACGCGGTTGGCGGTTGACCGCAAAGAGCGGGGCAAGGGTTTGGGCAAGCATCTGCTACG
TGATGCGATCGCGCGCTGTGTCGGGGTGGCCGAGCAGGTTGGGGTGCGGGCGATGCTTGTGCACGCATTGCACGACGAGG
CCCGCTCGTTCTACCTGCATTTCGAGTTCGAGCCTTCCCCGACTGATCCGCTGCACTTGATGCTCTCGATGAAAGACGCT
CGCGCGCTGATCGGTGCTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10416.03 Da
• Isoelectric Point: 6.7178

>AT36222 WP_085158599.1 NZ_AP022581:1062546-1062824 [Mycobacterium lacus]
MWCMATKTQRFAVRLTLEQDALIRHAAEVEGTDLTNFTVGATLAHARDVLADRRLFVLDNAAWAEFLAVLDRPVAHKPRL
EKLVAEPSIFER

Nucleotide

• Length: 279 bp

>AT36222 NZ_AP022581:1062546-1062824 [Mycobacterium lacus]
ATGTGGTGCATGGCTACGAAGACTCAGCGTTTCGCCGTGCGCCTGACCCTTGAGCAGGACGCGCTGATTCGTCATGCCGC
CGAAGTCGAGGGGACCGACCTCACCAACTTCACCGTCGGCGCGACGTTGGCGCATGCGCGTGACGTGCTGGCTGACCGCA
GGCTGTTCGTCCTTGATAATGCCGCGTGGGCGGAATTCCTGGCGGTGCTGGACCGGCCCGTCGCGCACAAGCCGCGGCTC
GAGAAGTTAGTCGCCGAGCCGTCTATTTTCGAACGCTGA