Detailed information of TA system TA036218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 409505..410135
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N24_RS01975
• Protein ID: WP_085156116.1
• Coordinates: 409698..410135 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: G6N24_RS01970
• Protein ID: WP_085156360.1
• Coordinates: 409505..409693 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS01960	405454..407037	+	1584	WP_085156122.1	serine hydrolase	-
G6N24_RS01965	407034..409415	+	2382	WP_085156119.1	HAD-IC family P-type ATPase	-
G6N24_RS01970	409505..409693	+	189	WP_085156360.1	antitoxin	Antitoxin
G6N24_RS01975	409698..410135	+	438	WP_085156116.1	SRPBCC family protein	Toxin
G6N24_RS01980	410240..411079	+	840	WP_085156113.1	VOC family protein	-
G6N24_RS01985	411046..411489	-	444	WP_085156110.1	cupin domain-containing protein	-
G6N24_RS01990	411701..412126	+	426	WP_085156357.1	hypothetical protein	-
G6N24_RS01995	412132..412629	-	498	WP_085156107.1	VOC family protein	-
G6N24_RS02000	412626..414140	-	1515	WP_085156104.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15941.62 Da
• Isoelectric Point: 10.3130

>T36218 WP_085156116.1 NZ_AP022581:409698-410135 [Mycobacterium lacus]
MAKLSGSIEVPLPPEQAWTHASDLSRYKEWLTIHRVWRSKLPDVLEKGAVVESIVEVKGMHNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIARVAPKDDRSVVSFDVHLGGPALFGPIGMIVAAALRSDIRESLQKFVTVFASS

Nucleotide

• Length: 438 bp

>T36218 NZ_AP022581:409698-410135 [Mycobacterium lacus]
ATGGCAAAGCTTTCCGGATCGATTGAAGTCCCGCTGCCGCCCGAACAGGCCTGGACGCATGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACGATCCACCGGGTTTGGCGCAGCAAGCTGCCCGATGTCTTGGAAAAGGGTGCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCACAACCGGATCAAGTGGACGATCGTGCGTTACAAGCCGCCGGAGGGCATGACGCTCAAC
GGCGATGGCGTCGGCGGTGTCAAGGTCAAGCTGATCGCCAGGGTCGCGCCGAAAGACGACAGGTCCGTCGTCAGCTTCGA
CGTGCATCTGGGTGGGCCGGCGCTGTTCGGCCCGATCGGCATGATCGTTGCCGCCGCGCTCCGAAGCGATATCCGCGAAT
CGCTGCAGAAGTTCGTCACCGTATTCGCCTCGTCCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6818.55 Da
• Isoelectric Point: 5.7808

>AT36218 WP_085156360.1 NZ_AP022581:409505-409693 [Mycobacterium lacus]
MGILDKAKDLLAQNADKVETVINKAGEFADEKTQRKYSDTIHKVQEQAKRVAGSDATGDQQN

Nucleotide

• Length: 189 bp

>AT36218 NZ_AP022581:409505-409693 [Mycobacterium lacus]
ATGGGAATCCTGGACAAGGCGAAGGACCTGTTGGCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAGGCCGGCGA
ATTCGCCGATGAGAAGACGCAACGCAAGTACTCCGACACCATCCACAAGGTGCAGGAGCAGGCCAAGAGGGTCGCCGGCT
CCGATGCGACGGGTGACCAGCAGAACTGA