Detailed information of TA system TA036217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 373614..374178
• Replicon: chromosome
• Accession: NZ_AP022581
• Organism: Mycobacterium lacus strain JCM 15657

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N24_RS01835
• Protein ID: WP_085156185.1
• Coordinates: 373614..373961 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N24_RS01840
• Protein ID: WP_085156182.1
• Coordinates: 373948..374178 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N24_RS01805	370132..370947	+	816	WP_085156202.1	DUF3027 domain-containing protein	-
G6N24_RS01810	370944..371399	-	456	WP_085156199.1	SRPBCC family protein	-
G6N24_RS01815	371444..371719	-	276	WP_085156196.1	DUF2530 domain-containing protein	-
G6N24_RS01820	371884..372315	+	432	WP_085156193.1	MarR family transcriptional regulator	-
G6N24_RS01825	372312..373166	+	855	WP_085156190.1	RNA methyltransferase	-
G6N24_RS01830	373163..373450	+	288	WP_085156188.1	DUF2537 domain-containing protein	-
G6N24_RS01835	373614..373961	-	348	WP_085156185.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N24_RS01840	373948..374178	-	231	WP_085156182.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6N24_RS01845	374311..375066	-	756	WP_085156179.1	DUF3071 domain-containing protein	-
G6N24_RS01850	375173..376291	-	1119	WP_139822173.1	phosphoserine transaminase	-
G6N24_RS01855	376542..377579	+	1038	WP_139822172.1	diiron oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-37)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12382.19 Da
• Isoelectric Point: 11.3028

>T36217 WP_085156185.1 NZ_AP022581:c373961-373614 [Mycobacterium lacus]
MLRGEIWQVDLDPARGGEANKRRPAVIVSNDRATAAATRLGRGVVTVVPVTSNTEKVYPFQVLLSGANSGLAVDSKAQAE
QVRSVATQRLLRRIGRVSPAELTDLDNALRLHLAL

Nucleotide

• Length: 348 bp

>T36217 NZ_AP022581:c373961-373614 [Mycobacterium lacus]
ATGCTGCGCGGTGAGATCTGGCAGGTCGACCTGGATCCAGCTCGCGGCGGCGAGGCGAACAAGCGGCGGCCGGCAGTCAT
CGTCAGCAACGACCGGGCCACCGCGGCCGCGACACGCCTCGGCCGCGGTGTGGTCACGGTTGTTCCGGTCACCAGCAACA
CCGAGAAGGTCTATCCATTCCAGGTCTTGTTGTCGGGGGCCAACAGTGGCCTCGCCGTCGATTCGAAGGCACAGGCCGAG
CAGGTCAGATCCGTCGCAACACAGCGCCTGTTGCGCCGAATCGGCCGAGTCTCCCCTGCCGAGCTGACCGATCTCGACAA
TGCGCTCAGGCTTCATTTGGCGCTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8260.10 Da
• Isoelectric Point: 4.2108

>AT36217 WP_085156182.1 NZ_AP022581:c374178-373948 [Mycobacterium lacus]
MKLSVSLSDEDVAALDAYVKRAGLPSRSAGLQRAVQMLRYPDLEDDYAEAWAQWSADECSAAWESTVGDGVTHAAR

Nucleotide

• Length: 231 bp

>AT36217 NZ_AP022581:c374178-373948 [Mycobacterium lacus]
ATGAAACTGAGCGTGAGCTTGTCTGACGAGGACGTCGCAGCATTGGACGCATACGTCAAGAGGGCGGGGCTGCCGTCGCG
GTCGGCAGGTTTGCAGCGTGCGGTCCAGATGCTGCGCTACCCCGACCTTGAGGACGACTATGCCGAGGCATGGGCACAGT
GGTCTGCCGACGAGTGTTCGGCTGCGTGGGAGAGCACCGTTGGAGATGGCGTGACCCATGCTGCGCGGTGA